KSR2 Gene Test Service
InquiryOverview
Genetics plays an important role in obesity and lipid metabolism disorders, and an increasing number of studies have addressed the genetic susceptibility of obesity and lipid metabolism disorders. Protheragen provides an Obesity Causation Analysis for obesity and lipid metabolism disorders to elucidate the pathogenesis of obesity and lipid metabolism disorders at the genetic level. The KSR2 gene plays an important role in energy uptake, energy metabolism, and insulin sensitivity. Variations in the KSR2 gene may lead to obesity and lipid metabolism disorders, and single-nucleotide polymorphisms (SNPs) in the KSR2 gene may be associated with obesity and lipid metabolism disorders.
Discover the Secrets of Genetic Obesity with Us: KSR2 Gene Testing
Our company uses reliable bioanalytical techniques to comprehensively analyze the role of the KSR2 Gene. Whether you want to explore the primary function of the gene or analyze it at an advanced level, our research team offers personalized lines of analysis.
Highly Efficient Sample Pre-processing
Based on a rigorous sample collection process, our skilled staff provides complete pre-sample processing, including DNA extraction and isolation, purity testing, and preservation services.
SNP Analysis
Based on the biological information related to the KSR2 Gene, we provide gene polymorphism site selection and site information analysis services.
- rs7955803, a synonymous mutation belonging to the coding region of the KSR2 gene.
- rs10774926 is located in the 3-terminal noncoding region of the KSR2 gene.
Genotype Analysis
Our company provides our clients with specialized genotype analysis using the classic genotyping method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Whereby our researchers use software to design primers and amplify them by PCR using DNA from polymorphic loci as templates. The PCR products are digested using restriction endonucleases, and gel electrophoresis separates the differently-sized fragments. We provide clients with clear typing results through the gel image analysis system.
Our lab provides efficient direct sequencing services to detect genetic changes. We use high-throughput sequencing to comprehensively cover the KSR2 gene profile.
Workflow
Our comprehensive analytical process includes genetic counseling, sample collection and transport, DNA extraction, gene sequencing, bioinformatic analysis, and data processing. Based on a strict DNA extraction process, we provide highly efficient sample collection and pre-processing services, which greatly reduce the sample pre-processing time. Our professional bioinformatics analysis team provides clients with visual gene mapping and data interpretation reports.
Applications
- KSR2 gene test provides insight into the complex relationship between obesity and bone health, and also provides new ideas for the treatment of bone diseases such as osteoporosis and myelolipomatosis.
- KSR2 gene polymorphisms serve as new targeted therapeutic loci for obesity and type 2 diabetes, providing new ideas for individualized medicine.
- The important role of the KSR2 gene in the regulation of AMP-activated protein kinase (AMPK)-mediated energy metabolism and insulin sensitivity. The KSR2 gene test plays an important role in the study of diabetes and other metabolism-related diseases.
Advantages of Us
- High-precision sequencing: Our company uses modern gene sequencing technology to accurately detect small changes in gene sequences, such as SNPs and insertions or deletions of small fragments (InDel), as well as large structural variants.
- Personalized service: Our company provides personalized gene sequencing services. According to the specific needs of clients, we customize suitable sequencing protocols and follow-up analyses.
- Professionalism and reliability: Our company has a professional gene sequencing team, including experienced technicians and specialized data analysts. With their profound professional knowledge and practical experience, they are able to ensure a smooth sequencing process and accurate interpretation of the results.
Our Anti-Obesity Services with Unlimited Possibilities
Publication Data
Technology: Real-time quantitative PCR, Western blot, Micro-computed tomography (micro-CT), X-radiation (X-Ray), Immunofluorescence imaging
Journal: Elife
Published: 2022
IF: 8.713
Results: The researchers used genetic editing techniques to observe and compare the differences in bone trabecular volume and bone marrow fat content between these models and wild-type animals by constructing animal models with overexpression and knockout of the Ksr2 gene. Meanwhile, the researchers used molecular biology and imaging techniques to examine and analyze the bones of experimental animals in detail. This study demonstrates that the obesity gene Ksr2 has distinct effects on bone trabecular volume and bone marrow adiposity. Overexpression of the Ksr2 gene leads to a decrease in bone volume, whereas Ksr2 knockdown increases bone marrow adiposity. These findings provide insight into the complex relationship between obesity and bone health.
Fig.1 X-ray measurements of Ksr2 gene editing mice. (Gomez, et al., 2022)
Frequently Asked Questions
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How does the KSR2 Gene affect adipogenesis?
KSR2 (Kinase suppressor of Ras2) protein is directly related to calcium store-manipulated calcium inward flow, and it plays an important role in the increase of cytoplasmic calcium concentration. KSR2 protein participates in the signaling pathway as a kinase component to enhance the cascade activation effect of the extracellular signal-regulated kinase (ERK) signaling pathway on calcium signaling. At the same time, the KSR2 protein connects and regulates the mitogen-activated protein kinase (MAPK) signaling pathway, an important energy receptor, which in turn interferes with and restricts the ERK signaling pathway. It has been shown that the KSR2 protein regulates the ERK signaling pathway and affects adipogenesis.
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How is the KSR2 Gene related to energy metabolism?
The KSR2 gene has an important regulatory role in AMPK-mediated energy metabolism and insulin sensitivity. The KSR2 gene promotes the action of AMPK, facilitates oxidative phosphorylation, and controls energy metabolism. The KSR2 gene plays an important role in the regulation of AMPK-dependent glucose uptake and oxidation of fatty acids in mouse neuronal cell lines. Disruption of AMPK-dependent regulation by knockdown of the KSR2 gene in living tissues affects the oxidative thermogenesis of fatty acids, which in turn leads to obesity.
Protheragen has an efficient sequencing process and rapid data processing capabilities to complete KSR2 gene sequencing and data analysis in a short time. This helps clients obtain sequencing results promptly and provides strong support for subsequent research. If you want to know more about the KSR2 gene, please feel free to contact us to explore together!
Reference
- Gomez, G.A.; et al. Contrasting effects of Ksr2, an obesity gene, on trabecular bone volume and bone marrow adiposity. Elife. 2022, 11: e82810. (CC0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.