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AMPK Pathway Functional Analysis Service

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Overview

The AMPK pathway is a core regulatory network for cellular energy metabolism and plays a key role in the pathogenesis of obesity, type II diabetes, and metabolic syndrome. The pathway regulates physiological processes such as glucose uptake, fatty acid oxidation, feeding behavior, and adipose tissue browning by sensing changes in the intracellular AMP/ATP ratio and is an important target for the treatment of metabolic diseases.

The AMPK pathway functional analysis service provided by Protheragen aims to provide precise data support and mechanism insights for the study of obesity pathobiology by deeply analyzing the AMPK signaling mechanism and revealing its role in energy balance, lipid metabolism, glucose homeostasis, and other physiological processes.

Fig.1 AMPK signaling pathwany. Fig.1 AMPK signaling pathways in obesity pathogenesis. (Wen, et al., 2022)

Targeting the AMPK Pathway to Unlock New Strategies for Obesity Intervention

AMPK Pathway Activity Assay

  • Our researchers provide professional phosphorylation site status analysis and pathway activation sensitivity analysis services. Moreover, our lab provides comprehensive regulatory network analysis services, including but not limited to kinases and effectors.

Metabolic Function Evaluation

  • Dynamic monitoring: Our professional analytical team provides clients with glucose transporter-4 (GLUT4) membrane translocation analysis, fatty acid oxidation rate measurement, and mitochondrial respiratory chain function assay.
  • Adipose tissue-specific analysis: With advanced analytical tools, our lab provides accurate quantification of brown fat thermogenic capacity and white fat browning markers.

Cross-Pathway Network Analysis

  • Relying on animal models and combining transcriptomic, proteomic, and metabolomic data, our company provides clients with cross-pathway network assessment and analysis.

Workflow

Schematic of AMPK pathway analysis. (Protheragen)

Applications

  • Our professional pathway functional analysis service helps clients to analyze AMPK-mediated feeding regulation (leptin/hungerin signaling) and energy dissipation mechanisms in adipose tissue.
  • Our pathway functional analysis service can be used to screen AMPK-specific agonists, optimize compound conformational relationships, and evaluate off-target effects.
  • Our pathway functional analysis service can be used to study the regulatory mechanisms of chronic inflammation, as well as aging and metabolism.

Advantages of Us

  • Accurate data delivery: We provide not only comprehensive raw data but also visual reports and processed charts.
  • Our research team encompasses experts from multiple disciplines, including metabolic pathology, computational biology, and medicinal chemistry, to design cross-pathway research programs for complex disease models (e.g., non-alcoholic steatohepatitis, insulin resistance).
  • From basic mechanism research to drug target discovery, our services cover all aspects of AMPK pathway research to meet the diverse needs of our clients.

Precise Analysis of the Mysteries of Obesity Metabolism

Publication Data

Technology: Immunoblotting, Liquid chromatography-mass spectrometry (LC-MS)

Journal: Cell Death & Disease

Published: 2021

IF: 8.1

Results: This study reveals that elaiophylin (Ela), a natural compound, exhibits robust AMPK activation capabilities. It demonstrates significant therapeutic potential in treating obesity and associated metabolic disorders through the modulation of multiple metabolic pathways. Specifically, Ela effectively reduces body weight and body fat content while improving glucose tolerance and insulin sensitivity in obese mice, with a favorable safety profile. RNA sequencing analysis indicates that Ela treatment upregulates the expression of genes involved in glucose utilization and fatty acid oxidation in hepatocytes. Additionally, gas chromatography-mass spectrometry (GC-MS) analysis confirms that Ela promotes glycolysis, the tricarboxylic acid (TCA) cycle, and amino acid metabolism pathways.

Fig.2 Elaiophylin activates AMPK in multiple cell lines. Fig.2 Ela activates AMPK in different cell models. (Bao, et al., 2021)

Frequently Asked Questions

  1. What are the metabolic effects of AMPK activation?

    When AMPK is activated, it acts as a key metabolic regulator in the four core metabolic areas in mammals: protein metabolism, lipid metabolism, sugar metabolism, as well as regulation of autophagy and maintenance of mitochondrial homeostasis, with a wide range of roles covering almost the entire physiological metabolic network of the living organism.

  2. How sensitive is the detection of the AMPK pathway? Can it detect low-abundance phosphorylated proteins?

    Our company uses high-sensitivity mass spectrometry to detect low phosphorylated proteins. For low-abundance samples, we offer pre-enrichment processing solutions (e.g., immunoprecipitation purification).

  3. Can we offer more to our clients with the help of bioinformatics technology?

    Sure! Relying on bioinformatics technology, we provide metabolic network construction and analysis, differential metabolite analysis, metabolic network visualization and interactive analysis, metabolic pathway optimization, and drug target discovery services.

Protheragen provides professional obesity signaling pathway functional analysis services to clients. We are ready to help you with your obesity research; feel free to contact us!

References

  1. Bao, R.; et al. Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK. Cell Death & Disease. 2021, 12(11): 972. (CC BY 4.0)
  2. Wen X.; et al. Signaling pathways in obesity: mechanisms and therapeutic interventions. Signal Transduction and Targeted Therapy. 2022, 7(1): 298. (CC BY 4.0)

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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