Adipocyte Fatty Acid–binding Protein Analysis Service
InquiryOverview of Adipocyte Fatty Acid–binding Protein (A-FABP)
At Protheragen, we are committed to providing our clients with the most cutting-edge and comprehensive biotechnology services. Our Services focus on various services such as Obesity Causation Analysis Services, Diagnostic Tests, Obesity Prediction Services, and especially Obesity Biomarker Identification and Analysis Services.
A-FABP, also known as adipocyte-type fatty acid binding protein, is a metabolic regulatory factor mainly secreted by adipocytes and macrophages. It is involved in intracellular fatty acid signaling and is closely related to a variety of metabolic and inflammation-related diseases. In obese individuals, the expression level of A-FABP is often significantly elevated, suggesting that it may be involved in the pathophysiological processes of obesity and related diseases.
Unlock Breakthrough Insights in Obesity Research with Our Advanced A-FABP Analysis Service
Our analysis is performed through a meticulous multi-step process designed to deliver scientifically robust and customizable insights.
Sample Collection & Preparation
- Our process begins with the collection of high-quality serum or tissue samples, ensuring consistency and purity for subsequent analyses.
- Samples are then prepared through standardized protocols that preserve the integrity of A-FABP for accurate quantification.
Quantification
Utilizing advanced mass spectrometry and enzyme-linked immunosorbent assay (ELISA) techniques, we quantify A-FABP levels. These technologies allow for the high sensitivity and specificity required to detect subtle changes in protein concentrations associated with obesity.
Custom Analysis Pathways
- We provide tailored analytical pathways based on unique research needs. This includes options for longitudinal studies, comparative analysis (e.g., age, BMI, gender), and correlation studies with other metabolic markers.
- Advanced statistical models are employed to interpret the data, identifying significant patterns and relationships with obesity-related phenotypic outcomes.
Data Interpretation & Reporting
- Our experts compile detailed reports that contextualize findings within current biomedical research.
- We ensure data is presented clearly, providing actionable insights and recommendations for further research or therapeutic interventions.
Consultation and Follow-Up
- To maximize the impact of our findings, we offer consultation services from our team of experienced biochemists and medical researchers.
- Clients are supported with follow-up services, facilitating the implementation of research outcomes into further investigative studies.
Workflow
Applications of A-FABP
- Study on the pathogenesis of obesity: A-FABP, as a key biomarker, can be used to study the pathogenesis of obesity further.
- Formulation of personalized intervention strategies: The level of A-FABP can be used as a reference for formulating personalized intervention strategies.
- Drug development and evaluation: A-FABP can be used as a drug target, which helps scientists develop drugs for obesity and related metabolic diseases.
Advantages
- High-precision detection: We use advanced biochemical analysis techniques to perform high-precision detection of A-FABP. These techniques have the characteristics of high sensitivity, strong specificity, and good repeatability, ensuring the accuracy and reliability of the test results.
- Comprehensive data analysis: We use bioinformatics tools to conduct a comprehensive analysis of A-FABP test data.
- Customized service: We provide customized A-FABP analysis services based on the specific needs of clients and sample characteristics.
Preclinical Studies of Anti-Obesity Therapeutics
Our adipocyte fatty acid–binding protein analysis service is integral to the preclinical studies of anti-obesity therapeutics, providing insights into the biomarker's role in fat metabolism. These studies, including Obesity Models, Pharmacodynamics, and Pharmacokinetics evaluations, as well as Bioanalysis and Safety Assessments, underpin the development of effective and safe treatments. Our expertise in these areas ensures robust preclinical data.
Publication
Published: 2005
Journal: Molecular & cellular proteomics
IF: 5.96
Results: The authors describe A-FABP as a small-molecular-mass protein that facilitates the intracellular transport of fatty acids between cellular compartments. It was identified as a potential biomarker for tumor progression in bladder cancer. This identification was based on observations of down-regulation of A-FABP expression in invasive urothelial carcinomas, suggesting a potential role in marking the progression of these tumors. Although the paper primarily studies its implications in cancer, this provides a foundational understanding of A-FABP as a biomarker in pathological conditions.
Fig.1 Illustration of A-FABP down-regulation in urothelial carcinomas. (Ohlsson, et al., 2005)
Frequently Asked Questions
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What is A-FABP?
A-FABP, also known as adipocyte fatty acid binding protein, is an important cytosolic protein of mature adipocytes. It belongs to the large family of fatty acid-binding proteins (FABPs) and accounts for about 6% of the entire cellular protein. A-FABP plays a key role in the transport and metabolism of fatty acids and is essential for the normal function and energy balance of adipocytes.
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What sample types are required for A-FABP analysis?
When performing A-FABP analysis, we accept a variety of sample types, including serum, plasma, cell supernatant, tissue, etc. These samples can be used for A-FABP determination after sample collection and processing. We guide clients to correctly collect and process samples according to specific needs and experimental requirements to ensure the accuracy and reliability of the analysis results.
At Protheragen, our A-FABP analysis service represents a critical tool for researchers aiming to unravel the complexities of obesity and related metabolic disorders. Our commitment to customized and high-quality analysis empowers our partners to advance the frontiers of obesity research with confidence and precision. Please feel free to contact us for more details if you are interested in our A-FABP analysis services!
Reference
- Ohlsson, G.; et al. Loss of expression of the adipocyte-type fatty acid-binding protein (A-FABP) is associated with progression of human urothelial carcinomas. Molecular & cellular proteomics. 2005, 4(4): 570-581. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.