- Anti-Obesity Compound Library
- GPCR/G Protein-Targeted Compounds
- Immunology/Inflammation-Targeted Compounds
- JAK/STAT-Targeted Compounds
- MAPK-Targeted Compounds
- Membrane Transporter/Ion Channel-Targeted Compounds
- Metabolism-Targeted Compounds
- NF-κB-Targeted Compounds
- Microbiology/Virology-Targeted Compounds
- Neuronal Signaling-Targeted Compounds
- PI3K/Akt/mTOR-Targeted Compounds
- Oxidation-reduction-Targeted Compounds
- Proteases/Proteasome-Targeted Compounds
- Stem Cells/Wnt-Targeted Compounds
- Tyrosine Kinase/Adaptors-Targeted Compounds
- Ubiquitin-Targeted Compounds
Recombinant Adenovirus in Obesity and Energy Metabolism Research
InquiryOverview
Adenovirus (Ad) vectors are indispensable tools in modern molecular biology and translational medicine. With their non-enveloped structure and high stability, these vectors offer unique advantages, enabling efficient transduction of diverse dividing and non-dividing mammalian cell types. Crucially, as non-integrating vectors, adenoviruses deliver genetic material to the cell nucleus while maintaining a circular plasmid state. This enables high-level transient gene expression without altering the host genome. Their high titer capacity, broad host range, and robust transient expression characteristics make adenoviral vectors the preferred platform for acute functional studies and rapid target validation within complex biological systems.
Strategic Gene Delivery for Metabolic Health
The global obesity epidemic demands sophisticated high-throughput tools to decipher complex metabolic signaling pathways and validate novel therapeutic targets. To address this need, Protheragen introduces adenovirus Particle Products designed to accelerate translational research in obesity. Our premium adenovirus particles enable high-level transient expression of target genes, supporting the establishment of reliable obesity models and rapid mechanism studies. These vectors exhibit high infectivity and efficiently transduce metabolism-related tissues, including liver, adipose tissue, and muscle.
Features
Protheragen employs industry-leading technology to produce the highest-quality adenovirus particles for gene overexpression and silencing studies. Our core platform is built upon a replication-deficient, E1/E3-deleted adenovirus serotype 5 (Ad5) backbone—the gold standard for achieving potent in vivo and in vitro gene delivery.
- Functional Genomics Payload Versatility
The Ad5 backbone offers exceptional flexibility, efficiently delivering large overexpression constructs (e.g., long cDNAs for secreted proteins) or gene silencing constructs (e.g., shRNA/miRNA expression cassettes) to support full-spectrum gain-of-function and loss-of-function studies.
- Robust transient expression
Following infection, the adenovirus genome remains in a circular state, ensuring high-level expression of obesity genes without the risk of genomic integration. This is crucial for studies requiring well-defined short-term functional perturbations.
- High-Efficiency Production
We utilize optimized HEK293 cell lines and strictly controlled bioreactor conditions to achieve maximum yield. Based on this, we scale production from pilot batches to high-throughput preclinical levels, specifically optimized for obesity gene overexpression applications. We guarantee sufficient viral particle counts to ensure reliable in vivo efficacy.
- Stringent Quality Control and Analytical Testing
We provide comprehensive testing, including titer determination, sterility testing, endotoxin assay, and mycoplasma detection, ensuring products meet critical preclinical research requirements. Additional quality control services, as well as in vivo and in vitro transduction and research services, are available upon request.
Applications
- Integrated Functional Gene Modeling: Mouse or rat models of therapeutic gene overexpression, often paired with adenovirus-mediated knockdown studies (shRNA delivery), are rapidly established. Through this process, the necessity and sufficiency of the target in metabolic pathways are validated.
- Mechanistic Studies of Adipogenesis and Lipolysis: Downstream signaling pathways and transcriptional networks activated by the overexpressed gene in target tissues are explored using the Adenovirus system.
- Drug Target Validation: The therapeutic potential of a specific gene is confirmed by the Adenovirus product prior to small molecule or antibody development, following the validation of its efficacy in reversing or mitigating diet-induced or genetic obesity phenotypes.
Advantages
Unparalleled Experience
Our expert team, with over two decades of industry expertise, commits to delivering rigorous solutions and forward-thinking scientific guidance throughout your project lifecycle, ensuring your research trajectory remains on a precise course.
Reliable Consistency
Standardized, validated production processes guarantee batch-to-batch stability, safeguarding reliable and statistically significant experimental outcomes.
Proven Efficacy
Effectiveness is validated through successful delivery and overexpression of key metabolic regulators, inducing observable phenotypic changes in obese animal models.
Publication Data
Title: Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats
Journal: PLoS One, 2019
DOI: https://doi.org/10.1371/journal.pone.0210930
Summary: This study employed adenovirus-mediated overexpression of salt-inducible kinase 1 (SIK1) to investigate its role and mechanisms in hepatic glucose and lipid metabolism. Results indicate that SIK1 overexpression ameliorates hyperglycemia, hyperlipidemia, and fatty liver disease by downregulating phosphoenolpyruvate carboxylase (PEPCK), glucose-6-phosphatase (G6Pase), etc, while inhibiting gluconeogenesis and lipid deposition. This establishes SIK1 as a potential therapeutic target for type 2 diabetes and fatty liver disease.
Fig. 1 Analysis of pathological changes in rats. (Song, et al., 2019)
Customer Review
Product Meets Experimental Requirements
"We utilized Protheragen's adenovirus products to achieve transient gene overexpression and observe phenotypic changes. The delivered products perfectly met our requirements and ensured high transduction rates."—Dr. Ma** C*
Reliable Adenovirus Supplier
"Protheragen's ultra-pure adenoviruses are of exceptional quality, facilitating our modeling efforts and enabling a series of in vivo studies. Highly recommended."—Dr. A** Sh***
Frequently Asked Questions
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How is the product titer determined?
Our adenovirus preparation method ensures an extremely high survival rate of viral particles.
Functional titer assay: We perform gradient dilutions of the adenovirus and transfect HEK293 cells. We then specifically detect the adenovirus hexameric protein and assess the functional titer by measuring the number of infectious units.
Ultra-purified adenovirus: We rapidly and accurately estimate the physical titer of viral particles directly by measuring OD260.
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Are there specific storage or handling precautions?
Adenovirus particles remain stable when stored at -80°C in the recommended buffer. Avoid repeated freeze-thaw cycles, as these significantly reduce titer. Detailed handling instructions accompany each shipment.
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What is the delivery timeline for Protheragen custom obesity gene overexpression adenovirus particles?
Leveraging optimized, high-efficiency workflows, we offer industry-leading rapid delivery services. Specific timelines vary based on scale and complexity, but projects can commence immediately. Contact us anytime for real-time project estimates.
Protheragen is your trusted partner for high-quality, high-impact gene delivery solutions in obesity research. We provide researchers with reliable, high-purity adenovirus products to help unravel the complex mechanisms of obesity-related diseases. With exceptional product quality and unparalleled research support, we ensure your projects advance efficiently and reliably. Contact us today for more details.
Reference
- Song, D.; et al. Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats. PLoS One. 2019, 14(6):e0210930. (CC BY 4.0)
Recombinant Adenovirus Products
- Species: Human
- Target Gene: LEP
- Species: Human
- Target Gene: FTO
- Species: Human
- Target Gene: MC4R
- Species: Human
- Target Gene: POMC
- Species: Human
- Target Gene: LEPR
- Species: Human
- Target Gene: PPARG
- Species: Human
- Target Gene: UCP3
- Species: Human
- Target Gene: GHRL
- Species: Human
- Target Gene: ADIPOQ
- Species: Human
- Target Gene: ADRB3
