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Recombinant MMLV Retrovirus in Obesity and Energy Metabolism Research
InquiryOverview
Moloney murine leukemia virus (MMLV) has long served as a classic and foundational tool in the field of gene delivery. The MMLV system is renowned for its exceptional transduction efficiency in actively dividing cells, making it an ideal choice for in vitro cell models, stem cell differentiation studies, and long-term genetic manipulation of proliferative cells. This capability provides a powerful tool for investigating complex biological mechanisms.
High-efficiency MMLV-mediated Stable and Persistent Expression of Obesity Genes
The ability to precisely regulate gene expression is crucial for elucidating the molecular mechanisms underlying complex diseases such as obesity. Protheragen leverages the powerful capabilities of the MMLV retroviral vector system to achieve highly efficient delivery of integrant gene vectors. Our specialized MMLV Viral Particle products are specifically engineered for high safety, exceptional titer yields, and outstanding performance in relevant cellular models, making them ideal for diverse obesity research applications.
Features
- We employ a robust three-plasmid system to produce replication-deficient MMLV viral particles: a transfer vector (containing the target gene), an envelope plasmid (encoding VSV-G envelope), and a Gag/Pol packaging plasmid.
- From target gene selection to functional viral particle project design and vector construction, our expert team develops optimal vector strategies. This includes selecting the most suitable promoter for your obesity-related gene of interest (GOI) or designing highly efficient shRNA sequences to achieve potent gene knockout.
- Constructed plasmids are co-transfected into proprietary high-yield packaging cell lines. These cells function as miniature viral factories, assembling replication-deficient viral particles carrying the target gene. Purification and Concentration After collecting viral supernatants, multiple purification steps—including ultracentrifugation or chromatography—remove cellular debris and concentrate the virus. This yields MMLV retroviruses of varying titers and high purity. Every batch undergoes rigorous quality control, reliably supporting your in vitro cell transduction needs and high-standard in vivo research.
- All products undergo rigorous quality control, including validation of key parameters such as infectivity titer determination, mycoplasma contamination testing, sterility testing (or endotoxin analysis). Additionally, we possess extensive resources of mast cell models and animal models, enabling us to perform customized viral transduction and subsequent functional studies tailored to your specific research requirements.
Applications
- Obesity Research: Utilizing the MMLV system to study adipocyte differentiation through overexpression or silencing of key transcription factors, thereby elucidating the molecular regulatory mechanisms underlying obesity and metabolic disorders.
- Stem Cells and Reprogramming: Utilizing MMLV vectors for efficient delivery of lineage-determining factors to precisely regulate stem cell differentiation pathways, directing cell fate toward specific functional metabolic cell types (e.g., for transplantation or model construction), thereby advancing regenerative therapy development.
- Drug Discovery and Target Validation: Rapidly establishing cell line models with sustained knockdown or stable expression of target genes, serving as an ideal in vitro screening platform. This is crucial for high-throughput, efficient screening and validation of novel anti-obesity compounds, metabolic modulators, and target-specific small-molecule drugs.
Advantages
Stability and Persistence
Our MMLV system enables stable integration of target genes into the host genome, essential for modeling chronic diseases like obesity.
High Titer and Purity
Delivered MMLV viral particles exhibit exceptional high titer and research-grade purity. Rigorous quality control ensures minimal endotoxin levels, providing customized products with high transduction efficiency and compatibility for demanding in vitro and in vivo applications.
One-Stop Service
Leveraging our mature technical platform and years of expertise, we offer comprehensive, reliable services spanning vector design to applied research.
Publication Data
Title: Molecular mechanisms of retroviral integration site selection
Journal: Nucleic Acids Res, 2014
DOI: https://doi.org/10.1093/nar/gku769
Summary: This study delves into the mechanisms of obligate integration of retroviruses—including MoMLV and HIV-1 lentiviruses—into host cell genomes and their target site selection preferences. MoMLV favors strong enhancer and promoter regions of active genes, whereas HIV-1 preferentially integrates within gene bodies. The study highlights the molecular basis underlying this targeting difference—key interactions between viral integrase and specific host cofactors anchor the virus to chromatin regions bearing distinct epigenetic marks. Furthermore, nucleotide preferences at integration sites correlate with the integrase's ability to locally bend DNA. This work also explores methods to enhance the safety of retroviral vectors by modifying integration site selection. This provides information support for retroviral delivery research.
Customer Review
Exceptional Product Quality
"The MMLV vectors prepared by Protheragen demonstrate exceptional batch consistency and ultra-high titers, successfully avoiding the high cytotoxicity associated with traditional viral vectors. We utilized these vectors to introduce target genes into difficult-to-transfect proliferating cell lines, achieving stable, permanent gene integration and expression. This establishes a high-quality tool foundation for our next phase of long-term functional studies and in vivo experiments."—Dr. Chr*** Ha**
Excellent Transduction Efficiency
"Protheragen's MMLV product enabled us to efficiently transduce key genes into target cells, achieving transduction efficiencies exceeding 90% with ease. This allowed us to rapidly establish fully functional disease cell models, significantly shortening our drug screening cycles. We have already deployed these high-quality models in subsequent drug screening and functional genomics studies, achieving rapid and critical progress."—Dr. Jor** Da**
Frequently Asked Questions
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Why choose MMLV over lentiviral vectors for constructing metabolic cell lines?
For actively dividing cells (such as most standard metabolic cell lines, primary preadipocytes, or HEK cell systems), MMLV is the superior choice. It efficiently integrates genetic material during cell division, ensuring robust and stable expression. Its biosafety profile is favored in in vitro studies, as it does not transduce non-dividing cells, thereby limiting off-target effects. Contact us immediately to determine the optimal vector for your specific cell type.
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Is MMLV vector integration into the host genome safe for experiments?
Our MMLV vectors are replication-deficient, meaning they cannot spread beyond the initial transduction event. While integration sites are random, this provides critical stability for long-term functional studies. We employ third-generation safety features to minimize the risk of recombination events.
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Is your MMLV product suitable for in vivo injection studies in obesity animal models?
Our MMLV products are engineered and quality-controlled for high-efficiency in vitro and in vivo applications. Upon understanding your specific requirements, we will develop a tailored solution and discuss it with you.
Protheragen is your professional partner for achieving efficient gene regulation in obesity research. Our recombinant MMLV retroviral products enable gene overexpression and shRNA silencing, offering stability, high titer, and stringent quality control for diverse obesity studies. With decades of scientific expertise and a customer-centric service model, we ensure project success from initial vector design to final high-titer delivery. Contact us to customize your dedicated viral products.
Reference
- Kvaratskhelia, M.; et al. Molecular mechanisms of retroviral integration site selection. Nucleic Acids Res. 2014; 42(16):10209-10225. (CC BY)
Recombinant MMLV Products
- Species: Human
- Target Gene: LEP
- Species: Human
- Target Gene: FTO
- Species: Human
- Target Gene: MC4R
- Species: Human
- Target Gene: POMC
- Species: Human
- Target Gene: LEPR
- Species: Human
- Target Gene: PPARG
- Species: Human
- Target Gene: UCP3
- Species: Human
- Target Gene: GHRL
- Species: Human
- Target Gene: ADIPOQ
- Species: Human
- Target Gene: ADRB3
