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PCR Assay Kit for Obesity Research

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Precisely Decoding the Mysteries of Obesity Genes to Help Obesity Research

(Protheragen)

As a biotechnology company specializing in obesity research, Protheragen understands that a deep understanding of the complex mechanisms of obesity is critical to developing effective interventions. With years of experience in the field of obesity research, we are committed to providing researchers around the world with high-quality, reliable research tools to help them make breakthroughs in the field of obesity research.

Our PCR kits are highly sensitive and specific, customized for obesity research. They are used to accurately analyze gene expression changes associated with obesity and other critical molecular events. In addition, these kits help in understanding the microbiota and provide reliable data for obesity probiotic research.

Gene expression analysis: PCR technology enables rapid and specific amplification of DNA fragments in vitro, thus enabling quantitative analysis of obesity-related gene expression. Meanwhile, through amplification and quantification, the differences in gene expression levels between different samples are compared for further research on obesity.
Microbiota analysis: By designing primers specific to particular bacterial populations, PCR techniques amplify DNA fragments of these bacteria to confirm their presence. This is important for revealing changes in gut microbial diversity in obese individuals. In addition, this has also been used to assess the impact of anti-obesity interventions on the gut microbiota.

Gene expression analysis testing process. (Protheragen)

Applications

Research on the pathogenesis of obesity: PCR kits help to gain insight into the role of different genes in the development of obesity, as well as the microbiota associated with obesity.
Drug target screening and validation: PCR kits also play a crucial role in evaluating the effects of potential anti-obesity drugs on the expression of relevant genes.
Nutritional intervention studies: PCR assay kits are used to analyze changes in the microbiota. Ideas for health promotion and weight control are provided by testing the impact of each, developing a personalized diet and probiotic supplementation program on the balance of the gut microbiota.

Advantages of Our CLIA Kits

Excellent sensitivity and specificity: These kits use advanced primer design and optimization protocols to ensure that non-specific amplification is minimized and accurate and reliable results are obtained.
Fast and convenient operation process: These kits are carefully optimized to provide a standardized operation process, which reduces the number of steps and saves time.
Comprehensive technical support: Our professional technical team is ready to provide you with comprehensive technical support, including optimization of experimental protocols, interpretation of results, etc., to ensure the smooth progress of your experiments.

Publication

Flavan-3-ols/procyanidins have some role in reducing the risk of obesity and metabolic syndrome. In this study, researchers analyzed the effects of highly polymeric procyanidins (PP) on obesity and lipid metabolism in mice fed a high-fat/high-sucrose diet. Through real-time quantitative RCP, metabolomics analysis, gene sequencing, and other analyses, the results showed that PP reduced the body weight of the mice and decreased the levels of endogenous metabolites associated with insulin resistance and the Firmicutes/Bacteroidetes ratio. This suggests that PP has some anti-obesity effects, affects the intestinal metabolome and intestinal flora, and exerts a beneficial effect on metabolic homeostasis.

Fig.1 PP altered the cecum microbiome of obese mice. Fig.1 PP affects the cecum microbiome of mice. (Masumoto., et al., 2016)

This study provides an idea for preclinical research on anti-obesity therapies, including the comprehensive use of PCR analysis, sequencing technology, and other means to systematically explore the expression level of inflammation-related markers, the status of intestinal flora, and so on, in order to comprehensively assess the anti-obesity effect.

At Protheragen, we have a deep scientific background and rich industry experience. Our PCR kits have been validated in practical applications and have won wide acclaim and trust. We are committed to being your reliable partner on the road to obesity research and jointly promoting the progress of science. Please feel free to contact us to learn more about the PCR assay kit and start your research journey!

Reference

Masumoto, S.; et al. Non-absorbable apple procyanidins prevent obesity associated with gut microbial and metabolomic changes. Scientific reports. 2016, 6(1): 31208. (CC BY 4.0)

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