Enzyme Substrates

Overview

In obesity research, enzyme substrates play a crucial role as core components of enzyme-catalyzed reactions. They are not only involved in various metabolic pathways in the body but also directly associated with energy intake, storage, and consumption, thus affecting the weight and obesity status of individuals. The obesity-related enzyme substrates provided by Protheragen provide clients with a powerful tool to explore the metabolic mechanism of obesity.

Enzyme Substrate Products: A Key to Exploring the Mechanisms of Obesity Metabolism

Our obesity-related enzyme substrate products are carefully screened and are a highly purified series of biomolecules that are directly related to key enzymes in obesity metabolism.

Dehydrogenases

Obesity-associated lipase substrates are catalyzed for hydrolysis by lipase. These substrates are broken down by lipase into smaller molecules, such as fatty acids and glycerol, and these breakdown products are subsequently utilized or stored by the body.

Research Area

• Enzymatic substrate regulation in fatty acid synthesis and catabolism
• Enzymatic substrates in glucose metabolic pathways and obesity risk
• Key enzymatic substrates in cholesterol and lipid metabolism
• Enzyme substrate abnormalities and insulin resistance
• Enzyme substrate changes in obesity-related inflammation
• Cardiovascular disease and enzyme substrate metabolism imbalance

Fluorogenic Substrates

Publication

Applications

• Lipase activity assay: Enzyme substrates can be quantitatively analyzed by changes in fluorescent signals to study lipase activity and, thus, its role in lipid metabolism.
• Drug screening: Potential anti-obesity drugs can be screened using fluorescent substrates to detect the potency of lipase inhibitors.
• Biosensor development: Fluorescent substrate-based biosensors for real-time monitoring of lipase activity provide technical support for obesity treatment.

Advantages of Our Products

Frequently Asked Questions

1. Are lipase substrates suitable for all types of lipases?

   Not all lipase substrates are suitable for all types of lipases. Different lipases have different substrate selectivity, e.g., Aspergillus niger lipase prefers short-chain p-nitrophenol ester as substrate. Therefore, when choosing a substrate, it is necessary to match it to the characteristics of the target lipase.

2. Do fluorescent substrates require special instrumentation?

   Fluorescent substrates usually require equipment such as a fluorescence spectrophotometer or a fluorescence microscope. For example, fluorescence emission intensity is measured at a specific wavelength when using a fluorescent substrate.

Protheragen provides enzyme substrates with high specificity, sensitivity, and versatility. Meanwhile, our products are suitable for research in various fields, such as molecular biology, cell biology, biochemistry, etc., which provides strong support for research on the mechanism of obesity and its related diseases. Please feel free to contact us at any time.

Enzyme Substrate Products