- Anti-Obesity Compound Library
- GPCR/G Protein-Targeted Compounds
- Immunology/Inflammation-Targeted Compounds
- JAK/STAT-Targeted Compounds
- MAPK-Targeted Compounds
- Membrane Transporter/Ion Channel-Targeted Compounds
- Metabolism-Targeted Compounds
- NF-κB-Targeted Compounds
- Microbiology/Virology-Targeted Compounds
- Neuronal Signaling-Targeted Compounds
- PI3K/Akt/mTOR-Targeted Compounds
- Oxidation-reduction-Targeted Compounds
- Proteases/Proteasome-Targeted Compounds
- Stem Cells/Wnt-Targeted Compounds
- Tyrosine Kinase/Adaptors-Targeted Compounds
- Ubiquitin-Targeted Compounds
Recombinant MSCV Retrovirus in Obesity and Energy Metabolism Research
InquiryOverview
In the fields of molecular biology and gene therapy, efficiently and stably introducing exogenous genes into target cells represents one of the key challenges for achieving functional research and disease treatment. Among these, the murine stem cell virus (MSCV) serves as an ideal delivery tool for the stable expression of target genes in hematopoietic stem cells, embryonic stem cells, and other cell lines.
Unlocking Stable Gene Delivery in Metabolic Research
Protheragen offers premium MSCV retroviral products, precisely engineered for efficient gene delivery, facilitating the translation of genomic discoveries into functional biological insights. Our proprietary MSCV platform is optimized for exceptional performance in challenging cell types.
- Optimized Vector Skeletons for Customized Operations
We provide robust, customizable vector skeletons specifically engineered for two core applications in obesity research:
- Gene Overexpression (cDNA Delivery): Vectors carry various obesity- and metabolism-related genes, supporting stable, sustained expression of target proteins. Optional reporter genes (e.g., GFP, RFP) or selection markers are available.
- shRNA Knockdown (Gene Silencing): We construct precise short hairpin RNA (shRNA) vectors driven by potent internal promoters to achieve robust, stable gene silencing of metabolic targets. This technology is essential for deciphering regulatory networks governing adipocyte differentiation, energy homeostasis, and related processes.
- Virus Production
All MSCV Viral Particles are prepared using a triple-plasmid transient transfection system in optimized production cell lines. Following concentration, purification, and quality control (including titer determination, sterility testing, mycoplasma testing, etc.), high-quality MSCV viral particle products are ultimately delivered. Additionally, we offer complementary target cell transduction efficiency assessment and functional analysis services tailored to specific customer requirements.
Applications
- Metabolic Pathway Research: MSCV viral particle products are used to manipulate the expression of genes involved in glucose homeostasis, insulin sensitivity, and lipid metabolism in stem cells and other mammalian cell lines to analyze metabolic pathway alterations.
- Stable Cell Line Construction: MSCV viral particle products are used to create cell lines stably expressing metabolic regulators or shRNA for high-throughput screening of anti-obesity drug candidates.
- Immune-Metabolic Crossover Research: MSCV viral particle products are used for efficient in vitro or in vivo transduction of hematopoietic stem cells to investigate the role of T cells, macrophages, and other immune cells in obesity-associated chronic low-grade inflammation.
Advantages
Comprehensive Quality Assurance
Each batch undergoes meticulous quality control procedures, including titer determination, sterility testing, and mycoplasma detection, ensuring reliability for in vivo experiments and cell culture studies.
Expert Support and Validation
With over 20 years of specialized expertise, we guarantee optimized vector design, troubleshooting, and reliable product delivery. We provide end-to-end technical support and consultation services to ensure your research projects advance smoothly and efficiently.
Superior Transduction Performance
The MSCV vector exhibits high transduction efficiency, significantly expanding its applications in regenerative medicine, gene therapy, and immunology research.
Publication Data
Title: Cell therapy could be a potential way to improve lipoprotein lipase deficiency
Journal: Lipids Health Dis, 2017
DOI: https://doi.org/10.1186/s12944-017-0577-4
Summary: Researchers utilized the MSCV retroviral vector to introduce the Lipoprotein lipase (LPL) gene into three distinct in vitro cell lines (muscle, kidney, and preadipocytes). After confirming functional LPL expression in all cell lines through in vitro experiments, these transfected cells were injected subcutaneously into nude mice to assess their in vivo LPL secretion capacity. Results demonstrated that subcutaneous injection of LPL-overexpressing adipocytes (3T3-L1) significantly increased LPL activity in the adjacent muscle tissue of nude mice. This study provides data support for the subsequent development and optimization of this MSCV-based gene delivery cell therapy.
Customer Review
MSCV Products Ensure Data Reliability
"Protheragen's MSCV vectors truly offer unmatched stability. Using their products, we completed cell transduction experiments and obtained reliable, interpretable data."—Ms. Gr*** L**
High-Quality MSCV Products
"Protheragen excels in designing and manufacturing MSCV products. Their turnaround is exceptionally fast, delivering products that fully meet requirements and enabling us to efficiently construct cells for screening potential compounds."—Dr. So*** Ro***
Frequently Asked Questions
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What are the core advantages of MSCV for the specific obesity research?
MSCV's proprietary long terminal repeat (LTR) sequence resists transcriptional silencing mechanisms present in stem cells and other cell types. This ensures stable, sustained transgene expression, which is critical for long-term or in vivo metabolic studies.
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Can MSCV transduce non-proliferating cells?
MSCV performs best in proliferating cells. For non-proliferating target cells like terminally differentiated adipocytes, ensure cells are in a cyclotic state or stimulated for activity. We recommend consulting us regarding specific cell types to confirm the optimal vector strategy.
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What is the highest purity grade available for in vivo studies?
Our ultra-pure in vivo grade product is purified via sucrose gradient ultracentrifugation and undergoes rigorous endotoxin testing. This high-purity formulation minimizes toxicity, ensuring safe and efficient application in animal models.
Protheragen offers stable, high-titer recombinant MSCV retroviral products. Whether your project requires stable overexpression of obesity-related genes or efficient shRNA silencing, our commitment to ultra-high purity, comprehensive quality control, and expert guidance ensures your success from vector design to functional data. Welcome to contact us to discuss customized solutions.
Reference
- Wu, W.; et al. Cell therapy could be a potential way to improve lipoprotein lipase deficiency. Lipids Health Dis. 2017, 16(1):189. (CC BY 4.0)
Recombinant MSCV Products
- Species: Human
- Target Gene: LEP
- Species: Human
- Target Gene: FTO
- Species: Human
- Target Gene: MC4R
- Species: Human
- Target Gene: POMC
- Species: Human
- Target Gene: LEPR
- Species: Human
- Target Gene: PPARG
- Species: Human
- Target Gene: UCP3
- Species: Human
- Target Gene: GHRL
- Species: Human
- Target Gene: ADIPOQ
- Species: Human
- Target Gene: ADRB3
