Product Overview
Description:
AdipoUpX™ Human LEP MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle. It is professionally engineered for the specific and efficient stable overexpression of the human LEP gene. The vector structure is optimized for hematopoietic and stem cell types. This ensures stable, high-level gene integration and expression within these cell lines. This product is an ideal tool for establishing stable cell lines and is suitable for in-depth research in obesity and energy homeostasis. The viral system offers high flexibility for customizing functional components.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
LEP
Full Name:
Leptin
Gene Symbol:
OBS; OB; LEPD
Summary:
LEP (leptin) protein is a systemically circulating cytokine-like molecule whose biological origin is white adipocytes. It connects to the central nervous system via endocrine pathways, with its core function being to serve as a signaling sensor for body fat stores. Upon entering systemic circulation, LEP molecules bind to specific receptors (LEPR) within the central nervous system, triggering activation of downstream signaling pathways such as JAK-STAT. Its primary physiological effects include suppressing the feeding center and enhancing thermogenesis. Beyond this central metabolic regulation, LEP's functions extend to immunity, reproduction, angiogenesis, and skeletal health. Abnormalities in the LEP gene or its regulation constitute a key molecular basis for metabolic disorders in humans, including early-onset, severe obesity and type 2 diabetes.
