Desulfovibrio Colonization Studies
InquiryOverview of Desulfovibrio (DSV)
DSV is a sulfate-reducing bacteria (SRB) that uses sulfate as an electron acceptor to derive energy from organic matter. DSV is a major component of the SRBs in the gut microbiota of healthy humans and crosses the mucus layer of the gut and colonizes the intestine species and number of DSV in the intestinal tract change when the host suffers from different diseases. The metabolites produced during the growth of DSV are also relevant to the maintenance of human health. Therefore, a comprehensive study of DSV is necessary. Protheragen provides our clients with high-quality DSV colonization research services to meet different research needs.
Exploring DSV Colonization: the Gut Code for Health and Disease
Isolation and Characterization of DSV
- Our researchers isolate and characterize DSV from feces and provide strain morphology, desulfurization performance, simulated digestate tolerance, H2S metabolism capacity, choline metabolism capacity, and antibiotic susceptibility analysis services.
Modeling and Colonization
- In the construction of obesity animal models, we have rich experience and professional technology. According to the needs of clients, through high-fat diet, drug-induced, and other ways, we successfully construct stable and meet the requirements of the study of obesity animal models. Our model construction process is rigorous and detail-oriented to ensure that the model truly reflects the physiological and biochemical changes of obesity in animals.
- At the same time, we also provide DSV colonization research services. Our researchers use the right way to introduce DSV into sterile mice and monitor the changes. By utilizing advanced microbial sequencing technology and bioinformatics analysis methods, we explore the association between gut flora and obesity and provide strong support to our clients in unraveling the mysteries of the pathogenesis of obesity.
Professional Analysis
- Our company offers a full range of metric measurement services, including but not limited to body weight, lean weight, fat weight, kidney weight, spleen weight, liver weight, perirenal fat weight, epididymal fat weight, mesenteric fat weight, and body fat distribution. Meanwhile, our researchers provide efficient blood collection and biochemical assay services.
- We provide whole genome sequencing services to elucidate strain genetic evolutionary relationships, mutation loci, and differential gene analysis.
- We provide quantitative analysis of DSVs in different intestinal tracts and differential analysis of DSV counts.
Workflow
Applications
- Our DSV colonization studies can be used to explore the potential relationship between DSV and gut flora diversity and intestinal diseases.
- Our DSV colonization studies have a certain significance for revealing the pathogenesis of obesity and researching related drugs for the treatment of intestinal diseases.
- Our DSV colonization studies can be used to analyze community structure and overall gene distribution.
- Our DSV colonization studies can be used to screen and characterize different batches of DSVs.
Advantages of Us
- Our company has a team of experts in the fields of gut health and microbiomics to provide in-depth scientific insights and technical support for our clients' obesity research programs.
- Our data processing team has strong data analysis capabilities to process and interpret complex microbiome data to provide meaningful results for our clients.
- If clients are interested in the effects of DSV on cells, we provide suitable cell models and comprehensive analytical services to elucidate the effects of DSV supernatant on cell activity and cytokine transcript expression.
One-Stop Anti-Obesity Therapy Development at Protheragen
Whether you want to develop anti-obesity targets, develop anti-obesity therapies, or are interested in pre-clinical studies of anti-obesity therapies, Protheragen is your best partner. We offer a wide range of models to meet the needs of our clients in therapeutic development. We support our clients in customizing their research routes.
Publication Data
Technology: qPCR (quantitative polymerase chain reaction), 16s rDNA sequencing
Journal: Microorganisms
Published: 2023
IF: 4.1
Results: This article describes the relationship between DSVs in the gut and a variety of diseases, particularly their role in obesity and other metabolic syndromes. The study mentioned that a high abundance of DSVs was observed in obese patients with Prader-Willi syndrome. Increased DSV was also observed in patients with type 2 diabetes (T2D). In addition, DSV abundance was also higher in patients with gestational diabetes mellitus (GDM). It was found that colonization of sterile animals with DSV resulted in a significant reduction of Clostridium perfringens. DSV elevated the expression of lipid regulatory factors, which led to the uptake of lipid dysfunction, resulting in an obese phenotype.
Fig.1 DSV products with pathogenic potential. (Singh, et al., 2023)
Frequently Asked Questions
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What are the common tests for DSV counts?
Commonly used assays for DSV counts include traditional culture methods, PCR, and PCR-denaturing gradient gel electrophoresis (PCR-DGGE).
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What diseases are associated with both colonization and changes in DSV?
DSV colonization and changes have been associated with a variety of diseases, including bacteremia, inflammatory bowel disease (IBD), neurodegenerative diseases, autism, cognition, cancer, and metabolic syndrome.
Protheragen provides professional DSV colonization and change research services. Our researchers further help clients dissect the mechanisms of DSV in disease onset and change. Please select the program you are interested in and contact us for more information.
Reference
- Singh, S.B.; et al. Desulfovibrio in the gut: the enemy within? Microorganisms. 2023, 11(7): 1772. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.