Overview

Bacteroides are Gram-negative, non-spore-forming, anaerobic, and rod-shaped bacteria that are resident in human flora. These strains are important in maintaining intestinal health, promoting digestion and nutrient absorption, and enhancing human resistance. Our Obesity Microbiota Research is dedicated to exploring the link between microbes and human health. Protheragen is committed to providing our clients with a comprehensive perspective to gain a deeper understanding of Bacteroides colonization in the body and to elucidate its specific mechanisms of influence on the development of obesity. In addition, our research focuses specifically on the role of Bacteroides in regulating intestinal microecological homeostasis, meticulously assessing the need for Bacteroides colonization.

Mysteries of Intestinal Microecology: An In-depth Analysis of Bacteroides Colonization

Our researchers introduce Bacteroides, a commensal organism of human origin, into the intestines of germ-free mice to determine if and how the genetic requirements for colonization change over time. We collect cecum contents at 0.5, 1, 2, 4, 7, 14, and 42 days post-colonization and sequence bacterial RNA samples. Moreover, our researchers provide functional genetic requirement analysis during colonization.

We provide professional metabolite analysis services by gas chromatography-mass spectrometry with electron impact ionization (GC-EI-MS).

Our specialized research team provides differential analysis of gene expression at different times in the mouse intestine. We provide a comparative analysis of gene expression at consecutive time points to fully characterize the gene pathways expressed during the acute phase of intestinal adaptation. We determine carbohydrate utilization through genomic expression analysis at successive times.

Workflow

Applications

• Bacteroides colonization studies can be used to explore the efficacy and potential mechanisms of the lipid-lowering and anti-obesity properties of Bacteroides.
• Bacteroides colonization studies can be used to study the structure and diversity of the gut microbiota. Moreover, our specialized analysis of biometabolites promotes the regulation of host metabolic signaling pathways.
• Bacteroides colonization studies can be used to gain insight into host-microbe interactions and how inter- and intraspecies microbial interactions contribute to the adaptation process.

Advantages of Us

• Our researchers have many years of proven experience in the isolation and cultivation of strains of bacteria, which greatly shortens project cycles.
• To ensure the safety and effectiveness of flora transplantation, our company has established a healthy donor screening process and traceability mechanism, the use of advanced testing technologies and methods, etc. These measures effectively exclude the presence of pathogenic bacteria, drug-resistant bacteria, viruses, and other risk factors to ensure the safety of the transplanted intestinal flora.
• We provide a full-service system from strain testing, donor screening, and strain transplantation to follow-up tracking and evaluation.

Your Best Associate in Anti-Obesity Research

Protheragen has always been at the forefront of anti-obesity therapeutic research, and our services include anti-obesity target development, anti-obesity therapeutic development, and a series of preclinical research services. Our services are deeply integrated with deep insights into the pathogenesis of obesity, cutting-edge drug discovery and development concepts, and a series of precision-targeted, high-efficiency anti-obesity therapeutic programs have been crafted. Through these efforts, we are committed to leading a new chapter in anti-obesity therapies.

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Publication Data

Frequently Asked Questions

1. What method details are included in this service?
   • Transcriptomic experiments
   • Genome-wide mutant fitness assays
   • Isolation of mutants from fecal matter
   • Isolate genome sequencing
   • Metabolite analysis using GC-EI-MS and methoxyamine and trimethylsilyl derivatization
   • Quantitative reverse transcription polymerase chain reaction (RT-qPCR)
   • Enzyme-linked immunosorbent assay (ELISA)
   • Histological procedures
   • Immunostaining and imaging
2. What is the link between Bacteroides and obesity?

   Bacteroides provide degradation and digestion work mainly for plant foods in the intestines. Bacteroides influence the process of digestion and absorption of food, thereby regulating the body's energy metabolism. As the number of Bacteroides in the gut decreases in obese people, it may lead to an increase in the absorption of fats and carbohydrates from food, which in turn increases energy intake and promotes the development of obesity.

Relying on advanced technology, Protheragen provides the most reassuring Bacteroides colonization research services to clients all over the world. Our comprehensive analysis helps advance targeted obesity interventions. No matter what problems you find in Bacteroides colonization research, please feel free to contact us for a prompt solution.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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