Overview of Resistin

Obesity not only affects one's body shape and appearance but is also closely related to a series of serious health problems, such as type 2 diabetes, hypertension, dyslipidemia, etc. At Protheragen, we are committed to providing precise solutions for various health problems through advanced biotechnology. In the broad service system of Our Services, Obesity Biomarker Identification and Analysis Services is one of our proudly featured services. With the rapid development of modern technology, more and more biomarkers are closely related to the occurrence and development of obesity, and resistin is one of them.

Resistin is an adipokine predominantly synthesized by monocytes and macrophages in visceral adipose tissue. It is associated with a range of metabolic and inflammatory diseases. In obesity, circulating levels of resistin are known to increase, contributing to chronic inflammation and insulin resistance. This exacerbates the pathogenesis of obesity-related disorders.

Discover the Pathway to Wellness with Precision Resistin Analysis

Sample Collection and Processing

• Sample types: We accept various types of biological samples, including blood, tissue, urine, etc. The specific sample type can be selected based on client requirements and experimental design.
• Sample processing: Collected samples undergo rigorous preprocessing steps, including centrifugation, serum/plasma separation, cell lysis, etc., to ensure the quality and stability of the samples.

Resistin Analysis

We employ advanced detection techniques to quantitatively measure resistin in samples. The specific detection method is selected based on client needs and experimental conditions.

• Enzyme-linked immunosorbent assay (ELISA)
  ELISA utilizes the specific binding reaction between antigen and antibody, converting a colorless substrate into a colored product through the action of an enzyme to determine the content of resistin in the sample. ELISA is characterized by high sensitivity, strong specificity, and ease of operation.
• Radioimmunoassay (RIA)
  RIA uses radiolabeled antigens to competitively bind with resistin in the sample to a limited amount of antibody, measuring the content of resistin by assessing the intensity of the radiolabeled isotope. RIA is known for its high accuracy.
• Real-time fluorescent quantitative PCR (RT-qPCR)
  RT-qPCR converts resistin's mRNA into cDNA through reverse transcription, then monitors the changes in fluorescent signals during PCR amplification in real-time using fluorescent dyes or probes to determine the expression level of resistin mRNA. RT-qPCR is highly sensitive and specific, accurately reflecting the expression status of the resistin gene.
• Western blotting (WB)
  WB detects the content and molecular weight of resistin protein in samples through specific antibody binding, electrophoresis, membrane transfer, and staining. WB directly measures protein levels and distinguishes between different molecular weights of resistin proteins.
• Mass spectrometry (MS)
  MS separates, ionizes, and detects resistin in samples, determining its molecular weight, structure, and content based on mass spectrometry information. MS is highly accurate and sensitive, but requires complex operation and expensive equipment.

Customized Analysis

• Personalized solutions: We offer customized resistin analysis solutions and design personalized experimental procedures and data analysis methods based on specific client needs and experimental objectives.
• Multi-omics analysis: Combining genomics, transcriptomics, proteomics, and other multi-omics data, we comprehensively analyze the expression level of resistin to gain a more comprehensive understanding of its role in obesity-related diseases.
• Dynamic monitoring: For clients requiring long-term monitoring, we provide regular resistin testing services to track disease progression and treatment effectiveness.

Reporting and Consultation

• Experimental report: We provide clients with detailed experimental reports, including sample information, detection methods, data results, statistical analysis, etc., to enable them to fully understand the resistin analysis results.
• Professional consultation: Our research team offers professional consultation services to address client inquiries regarding experimental design, data analysis, and other aspects, providing targeted advice and guidance.

Workflow

Applications of Resistin Analysis

• Exploration of obesity mechanisms: We are committed to revealing the specific mechanisms of obesity and its development through in-depth research, regulation, and functions of resistin, and we provide new ideas for the prevention and treatment of obesity.
• Obesity risk assessment: We use resistin as a biomarker, combined with clinical data and individual differences, to develop obesity risk assessment models.
• Obesity-related complications prediction: Resistin is not only related to obesity itself but also closely related to various obesity complications such as diabetes and cardiovascular diseases. We establish a prediction model by studying the relationship between resistin and these complications.

Advantages

• Advanced testing technology: We use a variety of advanced detection technologies, including ELISA, RIA, RT-qPCR, WB, and MS, to ensure the accuracy and sensitivity of resistin detection.
• Rich sample types: We accept a variety of biological samples, including blood, tissue, urine, etc., to provide clients with more diverse testing options.
• Professional scientific research team: We have a scientific research team composed of senior scientists and engineers. They are committed to technological innovation and scientific research cooperation to provide clients with the most cutting-edge scientific research results and solutions.

Anti-Obesity Therapy Development

Our resistin analysis service plays a crucial role in anti-obesity therapy development by offering insights into the biomarker's involvement in metabolic dysregulation and inflammation. Given the rising prevalence of obesity and its associated health risks, the development of effective anti-obesity therapies is paramount. Our comprehensive approach ensures that we are at the forefront of addressing this global health challenge, striving to improve the lives of those affected by obesity.

Publication

Frequently Asked Questions

1. What is resistin? What is the significance of resistin analysis?

   Resistin is a protein hormone secreted by fat cells and other tissues, which plays an important role in regulating energy balance, insulin resistance, and inflammatory response. Resistin levels in metabolic diseases such as obesity, type 2 diabetes, and cardiovascular diseases are often higher. Resistin analysis helps understand the possibility of obesity risk and related complications. This is of great significance for formulating personalized health management plans, monitoring and treatment effects, and preventing the progression of diseases.

2. Does your service support the customized resistin analysis scheme?

   Yes, we pay great attention to the personalized needs of clients and provide customized resistin analysis solutions. Our scientific research team tailors the most suitable analysis methods and processes for you according to your specific needs, sample types, and experimental purposes.

   Customized resistin analysis schemes may include:

   • Specific analysis method selection: According to your sample type and experimental requirements, select the most suitable Resistin analysis method.
   • Personalized experimental design: According to your research purposes and expected results, design personalized experimental solutions, including sample processing, detection conditions, data analysis, etc.
   • Professional consultation and guidance: Our scientific research team provides you with professional consultation and guidance throughout the analysis process.

Protheragen provides clients with comprehensive, accurate, and reliable resistin analysis to provide strong support for the study of obesity diseases. Please feel free to contact us for more details if you are interested in our resistin analysis services!

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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