Overview of Visfatin

Visfatin, also known as pre-B cell colony-enhancing factor (PBEF) or nicotinamide phosphoribosyltransferase (NAMPT), is an adipokine emanating from adipose tissues. It has been implicated in obesity and inflammatory processes within the bloodstream. This adipokine exerts multifaceted biological roles, encompassing immunomodulation, catalysis of distinct cellular functions, and anti-apoptotic properties. A previous investigation has established visfatin's potential involvement in the pathophysiology of diabetes and its significance in the genesis of metabolic syndrome. Visfatin, as a biomarker, plays a critical role in metabolic processes that are closely linked to obesity. Therefore, Protheragen, an advanced company in the field of obesity research, conducts a visfatin analysis that is essential for a comprehensive understanding of the disease's pathophysiology and the evaluation of therapeutic interventions.

Preclinical Studies of Anti-Obesity Therapeutics

Elevate Your Biomedical Insights with Visfatin: Key to Obesity

We provide analysis services for various types of samples, such as saliva and serum. Utilizing the human visfatin enzyme-linked immunosorbent assay (ELISA) Kit, we apply a method based on competitive binding for assessing visfatin concentration in samples. The microplate, precoated with an antirabbit secondary antibody, undergoes a blocking step and subsequent incubation with anti-visitatin antibody. During this phase, both biotinylated visfatin peptide and peptide standard or targeted peptide in samples competitively interact with the visfatin antibody. The unbound biotinylated visfatin peptide then engages with streptavidin-horseradish peroxidase (SAHRP), catalyzing a color development reaction. The intensity of this colorimetric signal directly corresponds to the quantity of the biotinylated peptide-SAHRP complex and inversely to the visfatin peptide in the standard or samples, as a result of the competitive binding to the visfatin antibody. By constructing a standard curve with known visfatin peptide concentrations, we accurately calculate the visfatin peptide concentrations in the samples, expressing them in ng/ml.

Anti-Obesity Therapy Development

By measuring visfatin concentrations with high precision, our researchers better understand the underlying mechanisms of obesity and identify potential therapeutic targets. Building on the foundational data from our visfatin analysis, we have extended our expertise to the development of innovative anti-obesity therapy solutions, including:

• Anti-Obesity Small Molecule Drug Development
• Anti-Obesity Gene Therapy Development
• Anti-Obesity Cell Therapy Development
• Anti-Obesity Antibody Therapy Development
• Anti-Obesity Tissue Engineering Therapy Development
• Anti-Obesity Nanotherapy Development

Workflow of Visfatin Analysis

Applications

• Our visfatin analysis can help researchers evaluate the inflammatory status in various conditions, including cardiovascular diseases and rheumatoid arthritis.
• In drug development, research on the functions and signaling pathways of visfatin may provide targets for new drug therapies, particularly in the treatment of metabolic disorders and cancers.
• Visfatin analysis can also be valuable in clinical trials and epidemiological studies, assessing its distribution in different populations and its relationship with various health conditions.

Advantages

• We have specialized knowledge and skills in conducting accurate visfatin analyses, enabling us to provide reliable results that inform obesity research and treatment strategies.
• We integrate visfatin analysis into various stages of drug development, from preclinical studies to pharmacodynamics and pharmacokinetics, ensuring a thorough understanding of therapeutic efficacy.
• We utilize cutting-edge techniques, such as the human visfatin ELISA Kit, which enhances the precision and reliability of visfatin concentration measurements in diverse sample types.

Publication Data

Frequently Asked Questions

1. What are the other sources of visfatin?

   Visfatin can be found in various sources such as subcutaneous adipose tissue, skeletal muscle, liver, immune cells, cardiomyocytes, brain cells, and renal glomeruli.

2. Where is the visfatin gene located?

   The visfatin gene, situated at chromosomes 7q22.1 to 7q31.33, encodes a protein of 491 amino acids, with a molecular mass of 52 kDa, and has been conserved throughout evolutionary history.

The visfatin analysis service that Protheragen offers is indispensable in obesity research for its role in validating preclinical models, assessing pharmacodynamic and pharmacokinetic profiles, integrating bioanalytical data, and ensuring the safety of anti-obesity therapeutics. If you would like to obtain critical insights into metabolic health and treatment responses by analyzing Obesity Biomarkers, contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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