Oxidative Stress Biomarker Analysis Service
InquiryOverview
Obesity, a chronic disease influenced by multiple factors impacting mitochondrial metabolism, is intricately linked to oxidative stress. Research has shown that obesity elevates the risk of various conditions, including hypertension, diabetes, dyslipidemia, and non-alcoholic fatty liver disease. Among the core pathological mechanisms of obesity and its related metabolic disorders, oxidative stress stands out prominently. Given the strong connection between obesity and oxidative stress, assessing the levels of oxidative stress biomarkers is pivotal in unraveling the pathogenesis of obesity. Furthermore, comparing biomarker levels before and after weight loss provides valuable insights into the physiological changes associated with obesity management.
Unlocking Insights: Precision Analysis of Oxidative Stress Biomarkers for Research Excellence
As a leading company focusing on Obesity Bioresearch, Protheragen has a special Obesity Biomarker Identification and Analysis Service. We provide our clients with assays, including but not limited to Leptin, Adiponectin, oxidative stress biomarkers, etc., and help to solve all kinds of problems encountered in the research. We also help identify and analyze novel obesity-related biomarkers.
Reactive Oxygen Species (ROS) Level Assay
ROS is a core product of the oxidative stress process, and its level directly reflects the state of oxidative stress within the cell. We accurately assess ROS levels and provide reliable data on ROS levels.
Antioxidant Enzyme Activity Assay
Antioxidant enzymes are important components of the body's antioxidant defense system, and their activity levels reflect the body's antioxidant capacity. We provide a wide range of antioxidant enzyme assays, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), etc.
Lipid Peroxidation Assay
The formation of lipid peroxidation products is one of the consequences of oxidative stress and may lead to changes in membrane permeability, integrity, and fluidity and even to severe cytotoxicity. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are commonly used as markers of lipid peroxidation and for the detection of oxidative stress. We quantify lipid peroxidation products such as MDA, 4-HNE, etc.
Detection of Protein Carbonylation Levels
Protein carbonylation is a hallmark event of oxidative stress, leading to impaired protein function. We quantify protein carbonylation levels. This assay reflects the effect of oxidative stress on cell function and provides a strong basis for assessing oxidative stress and the efficacy of anti-obesity drugs.
DNA Damage Detection
The level of 8-hydroxy-2'-deoxyguanosine (8-OHdG) is the most researched biomarker of DNA damage and various diseases. We measure 8-OHdG levels and analyze the correlation with obesity.
Our Reliable Assay
- Enzyme-linked immunosorbent assay (ELISA): ELISA is a highly sensitive and high-throughput protein quantification technique that plays an important role in the study of obesity-related oxidative stress biomarkers. We utilize ELISA to detect oxidative stress biomarkers, including lipid peroxidation products, in each sample.
- Liquid chromatography with tandem mass spectrometry (LC-MS-MS): LC-MS-MS is often used to quantify oxidative stress biomarkers in biological samples with high sensitivity. Our LC-MS/MS assays have been optimized and validated for the sensitive analysis of a wide range of analytes or biomarkers.
- High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS): These two technologies are used to detect a wide range of oxidative stress biomarkers with a high degree of accuracy.
Applicable Sample Types
The service is applicable to serum, plasma, urine, adipose tissue, cells, cell lysates, organoid models, etc. We pre-treat and test for various oxidative stress biomarkers.
Workflow
In studies of oxidative stress related to obesity, comprehensive biomarker analysis services are utilized to assess the level of oxidative stress in different samples. After understanding the client's experimental needs, a detailed experimental plan is developed and analyzed using various techniques. The final data detected is used to analyze the correlation between obesity and oxidative stress in-depth, contributing to obesity research.
Applications
- Pathogenesis analysis: By detecting oxidative stress biomarkers in different samples, we help to analyze the pathogenesis of obesity and related metabolic diseases, and provide useful information for targeted therapy research.
- Disease risk prediction: Detection of oxidative stress biomarkers such as 8-OHdG provides a basis for assessing the risk of other complications.
- Evaluation of therapeutic efficacy: Detecting the dynamic changes of oxidative stress biomarkers after the intervention of anti-obesity drugs, in order to evaluate the efficacy of various drugs in the treatment of obesity and its related complications.
Advantages
- Comprehensive coverage: Our service covers a wide range of oxidative stress biomarkers and can provide clients with a comprehensive analysis of oxidative stress status.
- Evaluation of high-precision tests: We use a variety of advanced testing techniques and methods to analyze changes in oxidative stress biomarkers and ensure the accuracy and reliability of the test results.
- Customized services: This service also allows customization of the biomarker panel. Clients can choose from the biomarkers listed above or add other biomarkers, and we adjust the detection range or sensitivity as needed.
- Personalized reports: We provide personalized reports and analysis suggestions according to the specific needs of our clients to help them better understand the results and develop follow-up research plans.
- Professional consultation: Our expert team provides professional consulting services to answer any questions during the research process.
Anti-obesity Therapy Development Services
We also offer comprehensive anti-obesity therapy development services.
Publication Data
Technology: ELISA
Journal: Oxidative Medicine and Cellular Longevity
IF: 5.076
Published: 2019
Results: In this study, biomarkers of inflammation, oxidative stress and endothelial dysfunction were analyzed in multiple subjects, and associations between parameters were determined. The results of the study showed that urinary C-reactive protein (CRP) and 8-isoprostane were positively correlated with obesity measures and markers of inflammation, oxidative stress, and endothelial dysfunction. This study analyzed associations between inflammation, oxidative stress, and endothelial dysfunction biomarkers in healthy, overweight, and obese children to provide data to support childhood disease surveillance studies.
Fig.1 The subject work characteristics (ROC) curve showing the performance of different biomarkers. (Selvaraju, et al., 2019)
Frequently Asked Questions
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What is the obesity oxidative stress biomarker analysis service?
Our oxidative stress biomarker analysis service is an analytical service for obesity research designed to help researchers quantify and understand the oxidative stress biomarkers associated with obesity. We have high-throughput analytical capabilities to efficiently test a wide range of samples and provide detailed interpretation of the data.
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What is the turnaround time for the oxidative stress biomarker analysis program?
The oxidative stress biomarker analysis length depends on the number of samples, the number of biomarkers selected, and the complexity of the assay. If customized, the assay may take longer.
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How to submit samples for analysis?
- Contact us: First, communication should be initiated with the service provider to discuss your project and obtain a quote. This step is crucial for understanding the scope of work, costs, and any specific requirements for sample submission.
- Receive instructions: Once contact is established, you get detailed instructions on sample collection, labeling, and shipping. These instructions guide you through the entire process, ensuring your samples are prepared and transported correctly.
- Prepare sample: The protocols provided for proper sample collection and storage should be followed.
- Ship the sample: Finally, the sample should be sent using a temperature-controlled method of transportation, such as dry ice, to ensure that it remains stable during transit.
Protheragen understands the critical role of oxidative stress in obesity and related diseases. Therefore, we are committed to providing our clients with the highest quality oxidative stress biomarker analysis services to help them achieve more significant results in obesity research and therapeutic studies. Please do not hesitate to contact us to learn more about our services or to inquire about collaboration.
Reference
- Selvaraju, V.; et al. Urinary biomarkers of inflammation and oxidative stress are elevated in obese children and correlate with a marker of endothelial dysfunction. Oxidative Medicine and Cellular Longevity. 2019, 2019(1): 9604740. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.