Precise Insights into Tissues: Advanced Function Analysis for Obesity Research

Functional Analysis

• Tissue cell isolation and phenotyping: We isolate individual cellular components from tissues and analyze them for their phenotype.
• Functional assays: We offer functional assays, including analysis of lipidogenic differentiation capacity, cell migration capacity, and macrophage activity.
• Genetic analysis: Key genes involved in adipogenesis, lipid metabolism, insulin signaling, cytokine production, etc., are quantitatively analyzed in the isolated cells. This process includes the detection of the expression of each gene using RT-PCR.
• Single-cell sequencing analysis: We perform efficient analysis of individual cells in tissues, including cell population clustering analysis, differential gene expression analysis, etc., to resolve cellular heterogeneity.
• Obesity-associated factor detection: We perform precise detection of obesity-associated factors, including interleukin (IL), lipocalin, leptin, and so on.
• Metabolic analysis: We provide a detailed analysis of lipid metabolism, sugar metabolism, cellular energy metabolism, etc.
• Tissue imaging: Based on high-quality imaging systems, we comprehensively and systematically analyze complex cellular phenotypes and their interrelationships within tissues.
• Spatial transcriptomics: mRNA expression in tissue sections is spatially resolved to obtain a comprehensive roadmap of transcriptional activity within the tissue, helping to accurately resolve mRNA expression at the cellular level.
• Other types of analysis

Sample Requirements

We accept a wide range of sample types, and specific sample requirements (minimum tissue volume, cell count, preservation conditions, etc.) vary depending on the project selected.

• Adipose tissue (subcutaneous, visceral, etc.), liver tissue, etc.
• Cell line samples: e.g., hepatocytes, primary adipocytes, stromal vascular fraction (SVF) cells, common adipocyte cell lines (e.g., 3T3-L1), etc.
• RNA samples
• Other types of samples

Analysis Report

Based on a variety of bioinformatics analysis tools, upon completion of the project, we provide raw data and detailed analysis of the results, including but not limited to:

Workflow

This service supports flexible experimental customization and allows researchers the flexibility to choose a single analysis project or combine multiple analysis types according to specific scientific needs.

One-stop Anti-Obesity Research Support

Applications

• Obesity mechanism research: In-depth analysis of tissue cell analysis helps to understand the pathogenesis of obesity and provides reliable information for the design of targeted therapies.
• Anti-obesity strategy analysis: Tissue cell function is precisely analyzed through gene expression detection, tissue imaging, cellular energy metabolism analysis, etc., in order to assess the role of various strategies in the treatment of obesity and related diseases.
• Anti-obesity drug development: Tissue cell function analysis is used to screen and evaluate the effects of candidate anti-obesity drugs on adipocyte function. Meanwhile, multi-technique analysis also helps to discover new drug targets and facilitate the development of anti-obesity drugs.

Advantages

• We specialize in the field of obesity and have a deeper understanding of the studies, a wealth of assay experience, and optimized study protocols.
• Our analyses include both essential functional assays and multi-omics analyses. By integrating powerful bioinformatics analysis, we provide multidimensional data to support a comprehensive understanding of tissue cell function analysis under obesity conditions.
• We are very good research partners and provide professional consultation and support in experimental design, data interpretation, etc., to promote the success of your research project.

Publication Data

Frequently Asked Questions

1. How do you ensure data quality and reproducibility?
   • All assays and analytical experiments follow standardized procedures that have been rigorously validated and optimized.
   • Our laboratory staff and scientific experts have extensive experience in handling samples and performing complex analyses.
   • Our analytical instruments are industry-leading and regularly maintained and calibrated.
   • Methods and results are described in detail in the final report.
2. What is the approximate project cycle time for the obesity tissue cell function analysis?

   The project cycle for this analysis depends on the complexity of the experiment and the number of samples. Basic functional assays (cytokine assays, glucose uptake analysis, etc.) usually have a shorter turnaround time than complex histological analyses (e.g., scRNA-seq or spatial transcriptomics, which involves library preparation, sequencing, and in-depth bioinformatics analysis). During the initial communication phase, we include the analysis cycle time for each experiment.

Protheragen is committed to providing scientists with the analytical tools, experimental, and data support they need to address the critical issue of obesity. Please feel free to contact us to discuss your specific research needs. We customize the most appropriate analytical solution based on your experimental objectives and sample profile.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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• Satiety Research
• Sugar-related Molecular Metabolism Analysis Service
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