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Obesity Signaling Pathway Functional Analysis Service

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Unlocking Obesity Pathways: Precision Insights for Therapeutic Innovation

An in-depth understanding of the signaling pathways involved in the onset and development of obesity helps develop more precise ways to combat obesity. With this in mind, Protheragen offers a cutting-edge obesity signaling pathway functional analysis. We integrate multiple experimental techniques to provide a comprehensive view of obesity signaling pathways. Through the function of signaling pathways, we help to better understand the complex network related to obesity, explore the mechanism of obesity development, and design appropriate therapeutic strategies based on it.

Fig.1 Summary of signaling pathways involved in obesity mechanisms. Fig.1 Obesity-related signaling pathways. (Wen., et al., 2022)

Detection of Key Factors

We detect key factors in the signaling pathway related to obesity, which play important roles in regulating adipogenesis, appetite control, energy metabolism, etc. By detecting the expression level or activity status of these factors through western blotting and real-time quantitative PCR analysis, we gain a deeper understanding of the pathogenesis of obesity and provide a scientific basis for the treatment of obesity.

Detection of Downstream Factors

This process involves a detailed analysis of the downstream factors of the obesity signaling pathway through metabolomics, proteomics, and so on. Understanding the metabolite differences and gene expression differences among different samples helps explore the characteristics and mechanisms of Obesity Pathology and screen potential therapeutic targets.

Data Analysis

We utilize statistical methods, bioinformatics analysis, systems biology modeling, and other technical means to conduct in-depth analysis and interpretation of experimental data (gene expression difference analysis, metabolic marker analysis, etc.). This helps to determine the roles and regulatory mechanisms of individual molecules in the signaling pathway and to validate putative hypotheses. We also provide professional data interpretation and consulting services to help our clients gain an in-depth understanding of the test results and develop appropriate research or treatment strategies.

We mainly provide the following signaling pathway analysis services:

MAPK Pathway Functional Analysis Service

MAPK signaling pathways have been found to play key roles in appetite, adipogenesis, glucose homeostasis, insulin resistance, thermoregulation, and obesity. For example, Brown adipose tissue (BAT) reduces obesity by increasing energy expenditure and is regulated by MAPK signaling. We use techniques, such as high-throughput sequencing, protein interaction analysis, and signaling pathway activation and inhibition assays, to analyze the function of the MAPK pathway in detail and provide useful information for studying obesity and anti-obesity therapies.

PI3K/AKT Pathway Functional Analysis Service

The PI3K/AKT signaling pathway is involved in regulating key cell growth and proliferation regulators, and its aberrant activation promotes obesity. For example, this pathway plays a role in obesity by regulating appetite in both central and peripheral systems. We systematically identify the key molecules and regulatory mechanisms in the PI3K/AKT pathway and conduct in-depth studies to provide a scientific basis for the study of obesity mechanisms and the development of new anti-obesity drugs.

JAK/STAT Pathway Functional Analysis Service

The JAK/STAT pathway activates various growth factors, hormones, and cytokines, is associated with the melanocortin pathway, and regulates energy homeostasis. Abnormalities in the pathway contribute to obesity either directly or through interactions with other signaling pathways. For example, STAT3 deficiency leads to insulin resistance and increased expression of gluconeogenic genes. We provide a very professional JAK/STAT pathway functional analysis service. By analyzing the function and mechanism of the JAK/STAT signaling pathway, we explore the pathogenesis of obesity and promote the development of related therapeutic strategies.

TGF-β Pathway Functional Analysis Service

The TGF-β superfamily consists of TGF-β, growth and differentiation factors, activator/inhibitor, and muscle growth inhibitor. It is involved in the regulation of lipid metabolism, glucose homeostasis, and appetite regulation. The TGF-β signaling pathway has been demonstrated to be associated with obesity and affects adipogenesis and adipocyte differentiation. We investigate the function of the TGF-β signaling pathway in organisms, its mechanism of action, and its impact on the development of obesity. Through an in-depth analysis, we reveal the pathogenesis of obesity and provide effective information for developing therapeutic strategies.

Wnt/β-catenin Pathway Functional Analysis Service

AMPK has been found to play a key role in obesity by regulating insulin sensitivity, white adipose tissue browning, BAT thermogenesis, and food intake. We provide a one-stop AMPK pathway functional analysis service. This service includes analyzing the changes of key active factors in the AMPK signaling pathway, regulation of energy homeostasis, regulation of glucose and lipid metabolism, and so on. Through an in-depth analysis of the activation and regulation of this signaling pathway as well as its interactions with other signaling pathways, we provide a scientific basis and strategy support for the in-depth study of obesity and the development of anti-obesity therapies.

Wnt/β-catenin Pathway Functional Analysis Service

Activation/inhibition of the Wnt/β-catenin signaling pathway has been shown to lead to differential effects on the pathogenesis of obesity. We provide an in-depth analysis of the function of this signaling pathway in organisms. Using advanced experimental techniques and bioinformatics methods, we systematically investigate the interactions and regulatory mechanisms of multiple key molecules. Our goal is to reveal the important role of the Wnt/β-catenin signaling pathway in various physiological processes and explore its relevance to obesity and potential therapeutic targets, which ultimately provide reliable support for the study of obesity pathogenesis and the development of targeted therapies.

Anti-Obesity Therapy Development

In addition to pathway analysis, we also provide anti-obesity therapeutic development services targeting these signaling pathways.

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Anti-Obesity Therapy

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Preclinical Studies

Workflow

The process of our obesity signaling pathway functional analysis service. (Protheragen)

Applications

Identification of potential therapeutic targets: By analyzing signaling pathways associated with obesity, researchers identify key nodes and interactions that may be effective targets for therapeutic intervention.
Genetic and molecular mechanisms understanding: Our signaling pathway analysis service helps to understand the molecular mechanisms that lead to obesity, providing insight into the etiology and progression of the disease.
Support for biomarker discovery: The signaling pathway analysis helps to discover obesity biomarkers for early detection, diagnosis, and monitoring of obesity-related diseases.
Development of personalized medicine: Insights gained from the signaling pathway analysis are used to develop personalized therapies based on different obesity pathogeneses.

Advantages

The obesity signaling pathway analysis services we offer are customized to provide tailored insights and solutions to specific research needs.
Through comprehensive signaling pathway analysis, we understand the pathogenesis of obesity, identify potential therapeutic targets, and develop new obesity interventions.
All analyses are performed using state-of-the-art equipment and proven techniques to ensure accurate results.

Publication Data

Technology: Gene editing, Western blotting

Journal: Signal Transduction and Targeted Therapy

IF: 39.3

Published: 2022

Results: This article provides a detailed overview of recent advances in the pathogenesis of obesity, the signaling pathways associated with obesity, and their roles in glucose homeostasis, adipogenesis, chronic inflammation, thermogenesis, and regulation of food intake. Several anti-obesity drugs have been developed to target these signaling pathways. The deeper excavation of signaling will bring new inspirations for obesity and therapeutic research.

Frequently Asked Questions

What is involved in the functional analysis of obesity signaling pathways?

This involves studying six obesity-related signaling pathways, detecting related upstream and downstream factors, and analyzing the specific roles of these pathways in the pathogenesis of obesity, including adipogenesis, appetite regulation, insulin resistance, adipose tissue inflammation, thermogenesis, and energy expenditure. Their interactions and regulatory mechanisms are also analyzed in detail, thus providing a scientific basis for the prevention, diagnosis, and treatment of obesity.
Who can benefit from this service?

Researchers specializing in obesity and metabolic disorders can gain important insights from our service to enhance their research and treatment development.
How can I access this service?

We offer customized obesity signaling pathway functional analysis protocols to meet specific research needs. You can contact us through our website and tell us your experimental needs. Our service staff will contact you for the first time and start the communication about the relevant program.

Protheragen is committed to providing comprehensive one-stop functional analysis services of obesity signaling pathways to academics and biotech companies in the field of signaling pathways and obesity research. Our professional team will provide you with expert advice and support at every stage of your project. Please feel free to contact us for further details on signaling pathway analysis.

Reference

Wen, X.; et al. Signaling pathways in obesity: mechanisms and therapeutic interventions. Signal transduction and targeted therapy. 2022, 7(1): 298. (CC BY 4.0)

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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