Wnt/β-catenin Pathway Functional Analysis Service

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Overview

Obesity, as a global epidemic, not only leads to excessive accumulation of body fat, especially concentrated in key areas such as visceral white adipose tissue and subcutaneous white adipose tissue, but also triggers a series of serious complications closely related to obesity. The classical Wnt/β--catenin signaling cascade plays a central and indispensable role in organ development and the maintenance of physiological homeostasis. Variants or mutations in genes in the Wnt/β--catenin pathway have been significantly associated with the development of metabolic disorders in human beings, which provides a new perspective for an in-depth understanding of the pathomechanisms of obesity and its complications. Protheragen provides Wnt/β-catenin pathway functional analysis service to help clients reveal the mechanism of obesity development.

Fig.1 A simplified representation of precise modulation of canonic Wnt/β-catenin signaling. Fig.1 Precise regulation of Wnt/β-catenin signaling. (Chen & Wang, 2018)

Obesity Signaling Pathway Functional Analysis: Wnt/β-catenin Pathway

Focusing on the complex pathological state of obesity, our service delves into the specific regulatory mechanisms of the Wnt/β-catenin signaling pathway in different adipose tissues. In particular, how these tissues respond to and regulate Wnt/β-catenin signaling when stimulated by a high-fat diet, and how this process affects adipose tissue browning (i.e., the conversion of white fat to brown fat), which in turn affects energy metabolism and weight management.

Precise detection of the expression levels of key molecules

Relying on high-throughput sequencing, proteomics, and other cutting-edge biotechnologies, our company provides clients with comprehensive analysis services of the Wnt/β-catenin pathway, accurately detecting the expression levels of key molecules in the pathway, such as β-catenin and transcription factors.

Analyzing the activation state of signaling pathways

Using cellular assays and animal model assays, we provide services to assess the activity of the Wnt/β-catenin pathway and its impact on function.

Analysis of adipose tissue-specific regulatory mechanisms

Our company provides studies on the specific regulatory mechanisms of the Wnt/β-catenin signaling pathway in different adipose tissues, especially subcutaneous white adipose tissue versus visceral white adipose tissue. We are particularly interested in the differences in Wnt/β-catenin signaling in subcutaneous versus visceral adipose tissue and how high-fat diets affect these differences.

Target validation

Through drug intervention, gene knockout, or overexpression experiments, our company provides services to validate the mechanism of the Wnt/β-catenin signaling pathway in obesity.

Fig.2 Wnt/β-catenin signaling in adipose precursors and pre-mature/mature adipocytes.

Fig.2 Wnt/β-catenin signaling in adipose precursors and adipocytes at different times. (Chen & Wang, 2018)

Applications

Our specialized analytical services can be used to screen potential modulators of the Wnt/β-catenin pathway, providing new ideas for obesity research.
Wnt/β-catenin pathway functional analysis can be used to screen and evaluate potential drug targets against the Wnt/β-catenin pathway.
Wnt/β-catenin pathway functional analysis can be used to develop biomarkers based on the Wnt/β-catenin pathway.

Advantages of Us

Our specialized analytical team includes talented individuals from multiple disciplines who are committed to delivering solutions that satisfy our clients.
From gene expression assays to function validation, we provide our clients with comprehensive coverage of Wnt/β-catenin pathway studies.
Our company offers personalized service solutions to meet the needs of our clients at different stages of research.

Satisfactory One-Stop Obesity Research Program

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Anti-Obesity Targets

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Anti-Obesity Therapies

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Preclinical Studies

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Obesity Insightss

Publication Data

Technology: Oil red O staining, Cell viability and reactive oxygen species (ROS) assay, Lipid accumulation assay, Real-time quantitative polymerase chain reaction (PCR) analysis, Immunofluorescence assay

Journal: International Journal of Molecular Sciences

Published: 2019

IF: 4.9

Results: In this paper, researchers investigated the role of porcine microtubule affinity-regulated kinase 4 (MARK4) in the regulation of lipid metabolism in porcine placental trophoblast cells. It was found that MARK4 significantly promoted lipogenesis and inhibited the PPARγ pathway by activating the WNT/β-catenin signaling pathway. This process may be associated with placental lipotoxicity due to sow obesity, providing new molecular mechanistic insights for understanding the effects of sow obesity on placental function.

Fig.3 Activation of the Wnt/β-catenin pathway promotes lipid accumulation in pig primary trophoblast cells. Fig.3 Effect of Wnt/β-catenin pathway activation on lipid accumulation in primary pig trophoblast cells. (Tian, et al., 2019)

Frequently Asked Questions

How do you determine if the activity of the Wnt/β-catenin signaling pathway is abnormal?

We assess the activity of the Wnt/β-catenin signaling pathway by examining the phosphorylation status of β-catenin, ubiquitination levels, and the activity of transcription factors.
For which types of obesity studies is the service applicable?

We provide services for a wide range of obesity studies, including genetic obesity, metabolic syndrome, obesity-related diabetes, and more.

Relying on our professional bioinformatics team, Protheragen provides clients with professional functional analysis services and comprehensive data parsing. Our goal is to be your best assistant in the field of obesity research. Whatever your challenges in obesity research, feel free to contact us at any time!

References

Chen, N.; Wang, J. Wnt/β-catenin signaling and obesity. Frontiers in Physiology. 2018, 9: 792. (CC BY 4.0)
Tian, L.; et al. Molecular characterization of microtubule affinity-regulating kinase4 from sus scrofa and promotion of lipogenesis in primary porcine placental trophoblasts. International Journal of Molecular Sciences. 2019, 20(5): 1206. (CC BY 4.0)

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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