Obesity-related Lipid Metabolism Analysis Service
InquiryOverview
In an era of increasing health consciousness, a deeper understanding of the complex mechanisms of metabolism is essential for managing obesity and related metabolic diseases. Protheragen provides Obesity Pathobiology Research services to comprehensively assess the central role of Sugar-related Molecular Metabolism and lipid metabolism in regulating normal physiological functions and pathological states of the body. Our lipid metabolism analysis services include an exhaustive analysis of plasma-free fatty acid metabolism and cutting-edge analysis of ceramide metabolism, which together reveal the lipid metabolism network for you.
Crack the Lipid Metabolism Code With Us
Plasma Free Fatty Acid Metabolism Analysis
Free fatty acids, also known as non-esterified fatty acids (FFA/NEFA), refer to fatty acids that are not esterified with substances such as glycerol and cholesterol. Our company provides services for the determination of free fatty acid concentrations and species in plasma, and the analysis of the metabolic pathways and regulatory mechanisms of free fatty acids. Moreover, our researchers provide services for the assessment of the association between fatty acid metabolism and obesity and its related metabolic diseases.
Ceramide Metabolism Analysis
Ceramide is a biologically active sphingolipid with cell signaling properties. Abnormal ceramide metabolism is associated with the pathogenesis of obesity-induced insulin resistance and metabolic diseases. Our company offers ceramide concentration and distribution assays. Relying on comprehensive characterization, we provide an analysis of ceramide metabolism for metabolic diseases such as obesity and diabetes.
Model Building
- Our company has extensive experience in constructing models that simulate metabolic diseases such as diabetes and obesity caused by nutritional imbalance and metabolic disorders in humans. We have skilled researchers and state-of-the-art technology to meet our clients' research needs.
- Our company detects the activity of the mouse for 24 hours, analyzes the change of circadian rhythm, collects food consumption, O2 consumption, and CO2 exhalation to derive respiratory entropy, and analyzes the metabolic situation of the mouse.
Sample Collection and Analysis
- We provide reliable sample extraction, isolation, and purification services.
- Based on liquid chromatography-mass spectrometry (LC-MS) technology, our company qualitatively and quantitatively analyzes the lipids in biological samples and discovers the composition and content changes of the lipids in the organism.
- Through these lipid molecules, we determine the change rule of other biomolecules interacting with lipids, thus revealing the mechanism of lipid metabolism in regulating the physiology and pathology of the organism and various life phenomena.
Workflow
Applications
- Obesity-related lipid metabolism analysis is essential in studying the development of other metabolic disease processes and pathogenesis studies.
- Obesity-related lipid metabolism analysis can be used for anti-obesity targets and biomarker discovery.
- Obesity-related lipid metabolism analysis can be used for the identification of lipid substances in tissue cells and the study of the regulation of lipid function and metabolism.
Advantages of Us
- High-throughput analysis: Our company has many years of research experience and advanced analytical tools capable of analyzing multiple lipid species simultaneously, providing comprehensive metabolic profiles.
- Our company offers polar and non-polar lipid metabolism analysis to meet the needs of different clients.
- In addition to basic metabolic characterization, we offer differential metabolite analysis and functional enrichment, and annotation analysis.
Unlocking the Molecular Secrets of Obesity with Protheragen
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Publication Data
Technology: Quantitative real-time PCR (qRT-PCR), UV spectroscopy, Western blot, Sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE), Hematoxylin and eosin (H&E) staining, Enzyme-linked immunosorbent assay (ELISA)
Journal: Cell Death Discovery
Published: 2022
IF: 6.1
Results: In this study, researchers evaluated the effects of ACE2 on lipid metabolism and inflammatory responses by using the Obesity-related glomerulopathy (ORG) mouse model. It was found that ACE2 deficiency exacerbated renal injury and lipid deposition, whereas ACE2 overexpression had a protective effect. In addition, this study reveals the mechanism by which ACE2 attenuates lipid deposition and inflammatory responses in ORG through the NF-E2-related factor-2 (Nrf2) pathway.
Fig.1 Effects of ACE2 deletion. (Chen, et al., 2022)
Frequently Asked Questions
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What are the effects of obesity on lipid metabolism?
The effects of obesity on lipid metabolism are mainly reflected in three aspects:
- It leads to the underutilization of energy and triggers pathological changes in cells.
- It alters the release of adipokines, such as an increase in leptin, which affects metabolic function and a variety of pathophysiological processes.
- It increases cytotoxicity and inflammatory response and promotes the development of atherosclerosis and related diseases.
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What samples are commonly used for lipid metabolism analysis?
Commonly used samples include tissue, fecal and intestinal contents, serum, plasma, urine, cerebrospinal fluid, lymphatic fluid, amniotic fluid, bile, and cells.
Relying on advanced analytical tools, Protheragen provides comprehensive lipid metabolism analysis services to help clients fully track lipid metabolic pathways. No matter what problems you encounter in lipid metabolism analysis, please feel free to contact us!
Reference
- Chen, Y.Y.; et al. ACE2 deficiency exacerbates obesity-related glomerulopathy through its role in regulating lipid metabolism. Cell Death Discovery. 2022, 8(1): 401. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.