Ceramide Metabolism Analysis Service
InquiryOverview
Ceramides are products of fatty acid synthesis and sphingolipid catabolism and are central molecules in Lipid Metabolism. The accumulation of ceramides in obese or dyslipidemic individuals triggers tissue dysfunction and is associated with diabetes and cardiovascular disease. Therefore, an in-depth analysis of ceramides is essential. Protheragen provides accurate detection and quantification of ceramides.
Focus on Ceramide Metabolism in Obesity-Related Metabolic Diseases
Sample Pre-processing
- Our researchers provide specialized sample collection services, such as blood and adipose tissue. For the collected samples, our researchers provide a range of services such as extraction, isolation, purification, and derivatization.
- Our company often uses normal-phase liquid chromatography, reversed-phase liquid chromatography, and thin-layer chromatography (TLC) for the separation of ceramides.
Ceramide Detection
- Benzoylation-ultraviolet (UV) detection: We provide professional benzoylation derivatization service and ceramide quantification service using benzoylation reagent for pre-column derivatization with ceramide.
- Fluorescent labeling-fluorescent detection: Our researchers use a variety of fluorescent reagents to label ceramides to make them usable for fluorescence detection. Some of our commonly used labels include anthracene phenol-cyanide, 6-methoxy-alpha-methyl-2-naphthaleneacetic acid (NAP), and ortho-phthalaldehyde (OPA).
- Evaporative light-scattering detector (ELSD): In combination with high-performance liquid chromatography (HPLC), we offer accurate quantitative analysis of ceramides.
- Liquid chromatography with tandem mass spectrometry (LC-MS/MS): Our high-resolution LC-MS/MS analysis helps clients gain insight into the type, structure, levels, and binding ability of ceramides to other metabolites.
Workflow
Applications
- Ceramide metabolism analysis can be used to predict the severity of cardiovascular disease and can also be used to study the mechanisms of the development of metabolic diseases such as obesity.
- Ceramide metabolism analysis provides a new idea for the prevention and treatment of obesity complicated with diabetes mellitus.
- Ceramide metabolism analysis plays a key role in drug development for obesity-related metabolic diseases.
Advantages of Us
- Our company offers LC-MS/MS analysis with high resolution, sensitivity, and specificity for accurate identification and quantification of different types of ceramides.
- The HPLC-ELSD system offered by our company allows direct quantitative analysis of ceramides without derivatization, thus avoiding errors associated with the derivatization.
- Our benzoylation-UV detection service provides excellent quantitative results with high assay sensitivity down to the nanomolar level.
Unlock Our Comprehensive Obesity Research Program
Publication Data
DOI: 10.3390/nu15030703
Journal: Nutrients
Published: 2023
IF: 6.706
Results: This article focuses on ceramide metabolism and its relationship to a variety of health conditions, particularly obesity, diabetes, and metabolic diseases. Studies have shown that ceramide levels are typically higher in obese individuals and that ceramide accumulation is strongly associated with insulin resistance and fat accumulation. Ceramide metabolism is also often abnormal in diabetics, which may affect insulin secretion and action. This study provides an important reference for a deeper understanding of the pathogenesis of these diseases and the search for effective therapeutic strategies.
Fig.1 Effects of phytochemicals on ceramide metabolism. (Kim & Jeon, 2023)
Frequently Asked Questions
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What is the difference between Normal phase and reverse phase chromatography for the separation of ceramides?
Normal phase chromatography has certain advantages in separating various types of ceramides. Reversed-phase chromatography separates the same type of ceramides according to the difference in their carbon chain lengths and is suitable for the separation of ceramide fractions.
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What are the common ceramide test components?
Our company's high-quality ceramide analysis includes but is not limited to C16:0-ceramide, C18:0-ceramide, C18:1-ceramide, C20:0-ceramide, C22:0-ceramide, C24:0-ceramide, and C24:1-ceramide.
Protheragen provides comprehensive ceramide metabolism analysis to explore in depth the relevance of ceramide dysregulation to the pathogenesis of obesity-induced insulin resistance and metabolic diseases. Feel free to contact us for high-level solutions!
Reference
- Kim, E.; Jeon, S. The impact of phytochemicals in obesity-related metabolic diseases: focus on ceramide metabolism. Nutrients. 2023, 15(3): 703. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.