Overview

Free fatty acids, also known as non-esterified fatty acids (FFA/NEFA), refer to fatty acids that are not esterified with substances such as glycerol and cholesterol. When abnormal FFA metabolism leads to hyper-FFAemia, there is an unbalanced transport of FFA from adipose to non-adipose tissue. Ectopic deposition of lipids occurs within non-adipocytes, which leads to the development of obesity, especially abdominal obesity. Protheragen provides accurate FFA metabolism analysis to support a comprehensive understanding of the physiological and Pathological Effects associated with obesity.

Plasma FFA Analysis Highlights Specific Changes in Lipid Metabolism

Our company offers not only services for monitoring changes in FFA profiles but also for assessing the correlation between FFA levels and disease.

Sample Preparation

• Our company provides professional sample extraction and purification services. Our researchers have extensive operational experience in plasma collection.
• Our common metabolite extraction methods include solvent extraction, solid phase extraction, or protein precipitation.

Sample Profiling

• We provide high-quality FFA quantification services using high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), colorimetric methods, and nuclear magnetic resonance (NMR) spectroscopy.
• We provide specialized differential metabolite analysis, functional enrichment, and annotation analysis services. Moreover, our researchers uncover the mechanisms of lipids in various life activities through the relationship between changes in lipid metabolism and changes in physiological pathology.

Data Processing

• Our company has a well-trained bioinformatics team to help clients process experimental data, screen metabolic targets, and generate visualization profiles.

Workflow

Applications

• Plasma FFA metabolism analysis can be used to screen for differential metabolic markers and further understand the mechanisms of obesity genesis.
• Plasma FFA metabolism analysis can be used to characterize changes in lipid levels in sample environments under different stimuli.
• Plasma FFA metabolism analysis can be used to gain insight into specific functions of FFA in health and disease.
• Plasma FFA metabolism analysis can be used to measure the effect of anti-obesity drugs on lipid metabolism in the body.

Advantages of Us

• Our professional analytical team adjusts the analytical parameters according to the characteristics of the client's sample, such as sample volume, injection volume, extraction solvent, concentration, column temperature, column type, mobile phase composition, or detection wavelength.
• We have highly trained data processing teams, cutting-edge analytical tools, and repetitive processes to ensure data reliability and consistency.
• We provide FFA derivatization analysis services according to clients' needs. Derivatization reagents are synthesized and labeled according to the clients' needs.

One-Stop Obesity Solution

Publication Data

Frequently Asked Questions

1. Why choose FFA as a test indicator?

   When the body has abnormal lipid metabolism, with increased lipolysis, increased FFA production, impaired FFA oxidation, and diminished FFA re-esterification, its concentration exceeds the upper limit of normal levels, leading to hyper-FFAemia. Hyper FFAemia may also be associated with atherosclerosis, arrhythmias, coronary heart disease, malignancy, and rheumatic immune diseases. Therefore, compared with other lipid indicators, FFA reflects the body's metabolism in addition to an earlier pathological state of the body.

2. What are the common FFA tests that are included?

   The tests we offer include, but are not limited to myristate, palmitoleate, palmitate, linoleate, oleate , cisvaccenate, eicosapentaenoate, arachidonate and docosahexanoate, α-linolenate, dihomo-γ-linolenate, FFA ratio, n-3/n-6 ratio, de novo lipogenesis index.

Protheragen provides specialized FFA metabolism analysis services to help clients gain insight into the relationship between FFA changes and the development of obesity. We provide satisfactory one-stop service solutions from model construction, sample analysis, and metabolic biomarker screening to data interpretation for clients worldwide. Please feel free to contact us to customize your analysis route!

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Ceramide Metabolism Analysis Service