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Adipose Tissue Inflammation Analysis Service

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Overview of Adipose Tissue Inflammation

In the intricate tapestry of adipose tissue biology, the initiation and perpetuation of inflammation over time are orchestrated by dysfunctional adipocytes, which release pro-inflammatory adipokines, and the infiltration of immune cells derived from bone marrow, which amplify the inflammatory milieu through the production of cytokines and chemokines. Although this inflammation is characterized as low-grade, its deleterious effects on remote organ function are profound, serving as a critical driver of the pathological consequences of obesity.

Protheragen analyzes the inflammatory mechanisms in adipose tissue, which is critical in obesity research. We evaluate the dysfunction of adipocytes, the secretion of inflammatory factors (e.g., cytokines and chemokines), and the infiltration of immune cells (e.g., macrophages and T cells). The data generated by this service serve as a key starting point for understanding the molecular basis of obesity-related inflammation, which is essential for subsequent anti-obesity therapeutic development.

Unlocking Insights into Inflammation: Your Gateway to Advanced Adipose Tissue Research.

We provide a comprehensive adipose tissue inflammation analysis service designed to deepen the understanding of inflammation processes within fat tissue. Our service focuses on several key areas:

Immune Cell Composition Analysis

We quantify and analyze the various types of immune cells and their relative abundance in adipose tissue. This includes precise identification and quantification of macrophage subtypes (M1 and M2), T cells, B cells, dendritic cells, and others to assess changes in cell composition during inflammation.

Cell Phenotype Analysis

Beyond cell type identification, we delve into the phenotypic characteristics of these cells, such as the expression levels of inflammatory cytokines and changes in cell surface markers. This helps us understand the roles of different phenotypes (e.g., the shift from M2 to M1 macrophages) in the inflammatory process.

Molecular Signaling Pathways Research

We investigate changes in adipocyte function and signaling, including increased lipolysis, reduced insulin sensitivity, alterations in the profiles of signaling lipids released, and changes in adipokine profiles. This helps to elucidate the long-term impacts of AT inflammation on metabolic health.

Mechanism Exploration

To explore the initiating mechanisms of adipocyte-immune cell crosstalk in metabolic diseases like obesity, we offer a range of experimental designs. This includes gene knockout or overexpression experiments, the use of signaling pathway inhibitors, and other methods to uncover the molecular basis of inflammatory responses in conditions such as obesity.

Integrated Data Analysis

We integrate and analyze multi-omics data from gene expression profiles, proteomics, and metabolomics to provide robust data support for AT inflammation research.

Workflow of Adipose Tissue Inflammation Analysis

Workflow of adipose tissue inflammation analysis. (Protheragen)

Applications

  • Our service can be used to screen and validate potential anti-inflammatory drugs aimed at mitigating the adverse effects of adipose tissue inflammation in obesity.
  • Our service can be applied to improve disease models for obesity and related metabolic disorders, aiding in the development and testing of new treatments.
  • Our service is useful for identifying specific inflammatory pathways and molecular targets involved in obesity-related adipose tissue inflammation.

Advantages

  • We offer tailored analysis solutions, from experimental design and sample processing to data analysis, ensuring that we meet the specific goals of our clients.
  • We possess expertise in multi-omics integration, combining transcriptomics, proteomics, and metabolomics to obtain a holistic view of adipose tissue inflammation.
  • Leveraging advanced bioinformatics techniques, we extract crucial insights from large datasets.

Publication Data

DOI: 10.1186/s12916-022-02672-y

Journal: BMC Medicine

Published: 2022

IF: 3.5

Result: In this article, the author explores the complex relationship between obesity and inflammation in adipose tissue, particularly the pivotal role of visceral fat. The author details how the initial disruption of adipocyte metabolic homeostasis triggers a cascade of events, including adipocyte hypertrophy, immune cell activation, and matrix remodeling, which are initially protective. However, in the context of chronic overnutrition, these mechanisms become insufficient, leading to adipocyte death, senescence, and structural damage. This damage further recruits immune cells and promotes matrix remodeling and potential fibrosis, while the accumulation of senescent cells perpetuates the inflammatory state. Thus, the inflammation that pervades obese visceral fat initially represents a protective response intended to contend with the onslaught of surplus nutrients. Yet, over an extended period, this inflammation may inadvertently contribute to tissue damage, thereby underscoring the complex interplay between obesity and inflammation in adipose tissue.

Frequently Asked Questions

  1. What are the physiological abnormalities in obese organisms?
    • Metabolic abnormalities
    • Oxidative stress
    • Mitochondrial dysfunction
    • Immune dysfunction
    • Chronic low-grade inflammation
  2. What are the decisive factors for adipose tissue inflammation?
    • Infections
    • Gut microbiota
    • Nutrients
    • Physical activity
    • Genes

Protheragen offers a comprehensive Obesity Pathobiology Research Service that focuses on elucidating the inflammatory mechanisms associated with obesity. contact us if you want to know the dysfunction of adipocytes, the secretion of inflammatory factors, and the infiltration of immune cells by quantifying immune cell composition, analyzing cell phenotypes, investigating molecular signaling pathways, and exploring the initiating mechanisms of adipocyte-immune cell crosstalk.

Reference

  1. Kolb, H. Obese visceral fat tissue inflammation: from protective to detrimental? BMC Medicine. 2022, 20(1): 494. (CC BY 4.0)

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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