Plasma Ceruloplasmin Analysis Service
InquiryOverview of Relationship Between Ceruloplasmin and Obesity
The intricate pathophysiology of obesity implicates a profound association between adiposity-induced inflammation and the constellation of metabolic derangements and chronic diseases that accompany it. The proinflammatory cytokines, secreted by adipose tissue, are posited to augment the hepatic production of ISPs—molecules that are indelibly linked to cardiovascular disease (CVD) risk. Among the ISP family, ceruloplasmin stands out as a clinically significant indicator of systemic inflammation. Functionally, ceruloplasmin is a copper-laden glycoprotein enzyme, ferroxidase, pivotal in the oxidation of ferrous iron (Fe2+) to ferric iron (Fe3+), enabling its subsequent sequestration by transferrin. The observed elevation in plasma ceruloplasmin levels is unequivocally linked to obesity, suggesting a potential role as a biomarker for the condition. At Protheragen, we help clients better understand the underlying mechanisms of obesity-induced inflammation and its role in disease progression by quantifying ceruloplasmin levels.
Targets for Developing Anti-Obesity Therapeutics
By targeting the pathways that control ceruloplasmin, we also help clients develop therapies to reduce inflammation and improve metabolic health. Ceruloplasmin's role in oxidative stress suggests it could be a target for antioxidant therapies to reduce tissue damage.
Anti-Obesity Therapy Development
We measure ceruloplasmin levels to help evaluate how well a therapy is working. A decrease in ceruloplasmin levels indicates reduced inflammation and improved metabolic health. By monitoring ceruloplasmin, we identify which drugs are most effective for anti-obesity.
Preclinical Studies of Anti-Obesity Therapeutics
In our preclinical studies, analyzing ceruloplasmin levels in animals helps validate the effectiveness and safety of new drugs. Changes in ceruloplasmin levels provide insights into how the drugs work and help identify any potential side effects.
Investigate Obesity Insights with Plasma Ceruloplasmin Analysis
We offer an extensive service for analyzing both the concentration and enzymatic activity of ceruloplasmin in plasma samples. This comprehensive analysis aims to guarantee precision and trustworthiness in both clinical practice and scientific exploration.
Blood Sample Acquisition and Processing
Upon receipt, the client's blood samples are meticulously processed. We subject the samples to centrifugation to extract the plasma, which is then pooled and partitioned into multiple aliquots to serve various experimental needs.
Testing Ceruloplasmin Stability
To study ceruloplasmin stability, we perform several experiments:
- Some aliquots are used immediately for baseline measurements (T0).
- Others are frozen at -20°C, subjected to freeze-thaw cycles, or stored at 4°C and room temperature to assess the effects of storage conditions on ceruloplasmin activity.
Measuring Total Ceruloplasmin
We quantify total ceruloplasmin using an electroimmunoassay performed on appropriately diluted serum samples, employing a polyclonal antibody specific to ceruloplasmin. A dilution series derived from an enzymatically inactive serum sample of known ceruloplasmin concentration is prepared and electrophoresed on each gel to construct a standard curve. We plot the peak heights of the test samples against this curve to determine ceruloplasmin concentrations.
Ceruloplasmin Oxidase Activity Measurement
We measure ceruloplasmin oxidase activity by quantifying the oxidation of paraphenylenediamine to a purple product, which is measured spectrophotometrically. A mixture of serum, sodium acetate buffer, and paraphenylenediamine solution is incubated, with the substrate oxidation being monitored at 610 nm at 30-second intervals for 5 minutes. The rate of oxidation is determined from the change in optical density at 610 nm over this 5-minute period. A standard curve is generated using a serum sample of known ceruloplasmin concentration, and the oxidation rates of test samples are converted to ceruloplasmin concentrations using a conversion factor derived from this standard curve.
Data Analysis
Utilizing statistical instruments within Microsoft Excel, we interpret the data, computing average values and standard deviations for each test cohort. A one-tailed correlation test is employed to ensure consistency across different assays. This rigorous methodology guarantees that our service yields dependable and precise insights into ceruloplasmin levels and activity.
Workflow
Applications
- Our service can be used to monitor changes in ceruloplasmin levels over time, tracking the progression of obesity and its impact on cardiovascular disease risk and other metabolic disorders.
- Our service can be used in developing and researching treatments for obesity and related conditions, because ceruloplasmin levels can serve as an endpoint to evaluate the efficacy of new drugs that target inflammation or metabolic regulation.
- Our service can be used to investigate the pathophysiology of metabolic syndrome, a cluster of conditions that include increased blood pressure, high blood sugar, excess body fat around the waist, and abnormal cholesterol levels — all of which are linked to obesity.
Advantages
- We offer a comprehensive range of services for analyzing both the concentration and enzymatic activity of ceruloplasmin in plasma samples, ensuring accuracy and reliability in both clinical practice and scientific research.
- We offer detailed data analysis using robust statistical tools to ensure the consistency and validity of our results.
- We provide detailed and comprehensive reports that include all relevant data, methodologies, and interpretations.
Publication Data
DOI: 10.1371/journal.pone.0080274
Journal: PloS one
Published: 2014
IF: 2.9
Result: In this article, the authors discuss the link between obesity and the increased risk of developing specific forms of cancer, exploring the role of adipokines in this relationship. They conducted a bioinformatic analysis to identify novel adipokines overexpressed in the adipose tissue of obese individuals and in tumor cells from obesity-associated cancers. Through this analysis, they identified ceruloplasmin as a novel adipokine that is significantly up-regulated in both adipose tissue of obese subjects and in cancer cells from obesity-related cancers. The authors also found that ceruloplasmin, which is involved in angiogenesis and copper transport, is produced and secreted at increased rates in obesity, with adipose tissue contributing substantially to the total circulating ceruloplasmin level in obese individuals.
Frequently Asked Questions
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What is the structure of human ceruloplasmin?
The human ceruloplasmin protein manifests as a singular polypeptide chain, encompassing 1046 amino acids and adorned with 4 glucosamine oligosaccharides, boasting a relative molecular mass nearing 132 kDa. The architectural blueprint of the ceruloplasmin peptide chain is predominantly characterized by beta strands and beta turns, constituting roughly half of its structure, with a conspicuous absence of α-helical formations. This polypeptide chain is susceptible to proteolytic cleavage, yielding three distinct isomorphic units with relative molecular weights of 67 kDa (comprising 480 amino acid residues), 50 kDa (405 amino acid residues), and 19 kDa (159 amino acid residues). These units are intricately linked within the intact polypeptide chain through the mediation of single amino acid residues, specifically arginine (R) and lysine (K).
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In addition to obesity, what diseases are associated with ceruloplasmin?
- Type 2 diabetes
- Hyperlipidemia
- coronary heart disease (CHD)
Protheragen offers state-of-the-art, highly accurate and precise quantification of ceruloplasmin levels in plasma, leveraging advanced immunoassays and spectrophotometry to measure total ceruloplasmin and oxidase activity. If you obtain comprehensive data analysis, rapid turnaround times, customized reporting, and thorough quality control that cater to research needs, contact us.
Reference
- Arner, E.; et al. Ceruloplasmin is a novel adipokine that is overexpressed in adipose tissue of obese subjects and in obesity-associated cancer cells. PloS one. 2014, 9(3): e80274. (CC BY)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.