Overview

The PI3K/AKT signaling pathway is a key regulator of cell growth and proliferation and plays a critical role in the regulation of appetite, insulin signaling pathways, and thermogenesis through the central nervous system and peripheral tissues. Abnormalities in this pathway have been found to promote the development of obesity and are associated with insulin abnormalities. Given the central role of the PI3K/AKT pathway in obesity and its complications, the development of novel anti-obesity therapies targeting this pathway has become a major hotspot and trend in current biomedical research. Protheragen provides high-quality PI3K/AKT pathway functional analysis services based on its rich experience in the detection of Obesity Pathobiology. By exploring the changes in PI3K/AKT pathway activity and its impact on cell proliferation, metabolism, and other biological processes, we further understand the pathogenesis of obesity and analyze the efficacy and safety of various anti-obesity therapies.

Multi-omics Analysis - Exploration of the Correlation Between PI3K/AKT and Obesity

PI3K/AKT Pathway Functional Analysis

Through sophisticated experimental design and advanced assay technology, we provide one-stop PI3K/AKT pathway functional analysis. Our team of scientists and bioinformaticians has extensive practical experience in Obesity Pathway Signaling Analysis (PI3K/AKT pathway, MAPK Pathway, JAK/STAT Pathway, TGF-β Pathway, AMPK Pathway, and Wnt/β-catenin Pathway). We customize our solutions according to clients' experimental requirements and are ready to answer any questions. Specific services include:

• Sample processing

The samples are processed appropriately, such as extracting proteins and RNA, for subsequent testing. Our PI3K/AKT pathway analysis service is available for a wide range of sample types.

• Pathway functional analysis

We utilize a variety of histological methods for more robust analysis of biological samples, including genomics, transcriptomics, proteomics, and metabolomics.

• Gene expression analysis

We analyze various gene mutations and modifications in the PI3K/AKT pathway, such as the expression of downstream effector molecules (mTOR, p21, p27, etc.) by RNA sequencing and reverse transcription-polymerase chain reaction (RT-PCR).

• Protein analysis

PI3K/AKT pathway-related proteins are detected by enzyme-linked immunosorbent assay (ELISA), western blotting, etc., and protein-protein interactions are analyzed.

• Metabolic analysis

We use liquid chromatography-mass spectrometry (LC-MS) and other techniques to analyze the changes in upstream signaling, downstream signaling, and other metabolic parameters of the PI3K/AKT pathway, and to understand the differences between samples in detail.

• Data analysis

We statistically analyze the experimental data to compare the differences in PI3K/AKT pathway activity between the obese and control groups. Using databases such as Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and GO, we gain insight into the biological processes involved and reveal potential targets for obesity treatment.

Detection indexes include but are not limited to the following:

Anti-Obesity Therapy Development

We are actively exploring drug discovery or therapeutic strategies based on the PI3K/AKT pathway to provide new ideas and approaches for the prevention and treatment of obesity and related diseases.

• Therapeutic design

We design and synthesize small-molecule drugs with specificity and low side effects against obesity-related targets (e.g., key molecules in the PI3K/AKT signaling pathway). In addition, we are actively researching and developing cutting-edge anti-obesity therapies such as gene therapy and cell therapy with significantly enhanced efficacy, and are committed to providing comprehensive and in-depth research support to our clients.

• Preclinical Studies

We have a wealth of in vivo and in vitro obesity models and conduct detailed evaluations of anti-obesity therapies. Tests include behavioral observation, biomarker testing, physiological and pathological change analysis, gene expression analysis, etc.

Workflow

Functional analysis of the PI3K/AKT pathway is essential for in-depth investigation of the pathogenesis of obesity, identification of potential therapeutic targets, and development of new therapies. After detailed communication of the experimental requirements, we formulate a tailored experimental plan and have professional experimental personnel responsible for the completion of the entire experiment. At the end of the project, we process and analyze the data in detail and provide some valuable insights. Throughout the project, we maintain communication and answer any questions encountered during the study.

Applications

• PI3K/AKT pathway analysis plays a crucial role in studying obesity mechanisms and discovering new obesity-related biomarkers.
• PI3K/AKT pathway analysis helps to provide new ideas and targets for the development of obesity prevention and treatment methods.
• PI3K/AKT pathway analysis helps to evaluate the therapeutic efficacy and safety of various drugs or anti-obesity therapies in more detail.

Advantages

• The detailed functional analysis of our PI3K/AKT pathway provides a powerful tool for understanding the complex biological systems behind obesity and related complications.
• Our state-of-the-art experimental equipment ensures that the data from individual assays are flexible, highly sensitive, and reproducible. At the same time, advanced algorithms and computational resources ensure that the data are processed and analyzed in a complex manner.
• We maintain open communication and collaboration throughout the pathway analysis process and are always available to answer any questions our clients may have.

Publication Data

Frequently Asked Questions

1. How can PI3K/AKT pathway analysis facilitate the development of anti-obesity therapies?

   The PI3K/AKT signaling pathway regulates cell proliferation, differentiation, and metabolism, and is associated with a variety of diseases such as obesity, diabetes, and cancer. Targeted therapies for the PI3K/AKT pathway can help reverse the effects of pathway abnormalities and thus provide some anti-obesity effects. For example, pharmacological inhibition of PI3K is an effective and safe anti-obesity measure, which plays a certain role in reversing the negative effects. The analysis of the PI3K/AKT pathway plays an important role in analyzing the mechanism of obesity, discovering specific therapeutic targets, and developing targeted therapeutic strategies.

2. What is the cycle time for PI3K/AKT pathway functional analysis?

   The cycle time for PI3K/AKT pathway functional analysis varies depending on the technology and complexity of the experiment. After defining the experimental requirements, we will carefully plan the experimental program and detail the time required for each process. With a strict project management system, we ensure that we move forward efficiently and complete all experimental steps quickly.

At Protheragen, our high-quality PI3K/AKT pathway functional analysis service advances scientific research into the treatment of obesity and its related complications. Whether you want to explore the dynamics of obesity-related biological processes or identify potential therapeutic targets, we offer a one-stop customized service. Please feel free to contact us for more information about PI3K/AKT pathway analysis.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• JAK/STAT Pathway Functional Analysis Service
• MAPK Pathway Functional Analysis Service