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TGF-β Pathway Functional Analysis Service

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Overview

The TGF-β (transforming growth factor-β) signaling pathway plays a central role in cell proliferation, differentiation, apoptosis, and immune regulation, and its aberrant activation or inhibition is closely related to a variety of pathological processes, including obesity, metabolic syndrome, cardiovascular disease, and tumors. Protheragen systematically analyzes the molecular mechanism of the TGF-β pathway in obesity-related diseases through the integration of high-throughput sequencing, bioinformatics analysis, and functional validation technologies, providing a scientific basis for the study of disease mechanisms and the development of intervention targets.

Precise Analysis of TGF-β Pathway and Decoding of Obesity Pathogenesis

Comprehensive Testing of the TGF-β Signaling Pathway

  • Ligand-receptor binding assay: Our researchers provide expression level and activity assays for TGF-β1, TGF-β2, and TGF-β3 by enzyme-linked immunosorbent assay (ELISA), western blot, and other methods.
  • Activation analysis of transcription factors: Our professional analysis team detects the activation of Drosophila mothers against decapentaplegic protein 2/3 (SMAD2/3) and other transcription factors by ChIP sequencing (ChIP-Seq), quantitative polymerase chain reaction (qPCR), and other techniques.
  • Dynamic changes in intracellular signaling pathways: Our researchers provide dynamic changes in intracellular signaling molecules through flow cytometry, fluorescence microscopy, and other analyses.

Functional Research

  • Obesity and metabolic disorders: Our company provides analysis of the regulatory mechanism of the TGF-β signaling pathway in obesity models, helping clients to reveal its relationship with adipose tissue remodeling, insulin resistance, etc.
  • Cellular function verification: Our company provides evaluation services for the effect of TGF-β on adipocytes and immune cells.
  • Animal model validation: Our professional analytical team helps clients to clarify the mechanism of action of the TGF-β pathway in obesity complications. We provide constructive TGF-β pathway knockout/overexpression mouse model construction services, which are further combined with metabolic phenotyping (e.g., body weight, blood glucose, blood lipids) and histopathological testing.

Workflow

Schematic of TGF-β pathway analysis. (Protheragen)

Applications

  • TGF-β pathway functional analysis helps to study the role of TGF-β in obesity, diabetes, fibrosis, tumors, and other diseases and provides new ideas for the diagnosis and treatment of diseases.
  • TGF-β pathway functional analysis can be used to screen drugs targeting the TGF-β signaling pathway and develop novel anti-obesity drugs.
  • TGF-β pathway functional analysis can be used to develop pathway marker-based obesity diagnostic or prognostic assessment tools.
  • TGF-β pathway functional analysis can be used to screen TGF-β pathway-related targets and evaluate the efficacy and safety of drug candidates.

Advantages of Us

  • Our company combines transcriptomic, epigenomic, and proteomic data to reveal a panoramic picture of pathway regulation.
  • Our company adopts international advanced testing technologies, such as ELISA, ChIP-seq, flow cytometry, etc., to ensure the accuracy and reliability of the test results.
  • Our company provides a full range of assays from upstream to downstream of the signaling pathway, covering ligands, receptors, transcription factors, and downstream effects to help clients gain a comprehensive understanding of the function of the TGF-β signaling pathway.

Multi-Omics Integration Promotes Obesity Research

Publication Data

Technology: Flow cytometry, SA-β-galactosidase staining, DNA damage assay, Immunofuorescence staining, Scanning electron microscopy

Journal: Stem Cell Research & Therapy

Published: 2023

IF: 7.5

Results: This literature focuses on the effects of obesity on the remodeling of adipose-derived mesenchymal stem cells (hASCs) and their extracellular matrix (ECM). The results showed that obese hASCs exhibited significant dysfunction, including mitochondrial dysfunction and increased expression of senescence-related genes. Meanwhile, the ECM components secreted by obese hASCs were also altered, especially the expression levels of collagen I and TGF-β1 were significantly upregulated. These changes further affected the proliferation, differentiation, and metabolic functions of hASCs.

Fig.1 The properties of ECM in vWAT from lean and obese objects were determined. Fig.1 Determination of ECM properties in vWAT of lean and obese objects. (Han, et al., 2023)

Frequently Asked Questions

  1. How to choose the appropriate assay?

    Choose the appropriate assay according to the research objectives, such as ELISA for ligand levels, ChIP-seq for transcription factor activation, and flow cytometry for dynamic changes in intracellular signaling pathways. Our professional technical team provides the most suitable solution according to the client's project details.

  2. Are the mechanisms of the TGF-β signaling pathway in disease complex?

    Yes, the TGF-β signaling pathway has a complex regulatory mechanism, and it may exhibit either promotional or inhibitory effects in different diseases, so comprehensive experimental assays and analyses are needed to reveal its functions.

Protheragen provides professional Obesity Signaling Pathway Functional Analysis Service to clients. Our team consists of senior researchers with rich experience in signaling pathway research and provides professional experimental design and data analysis support. No matter what problems you encounter in signaling research, please feel free to contact us at any time!

Reference

  1. Han, X.; et al. Blockade of TGF-β signalling alleviates human adipose stem cell senescence induced by native ECM in obesity visceral white adipose tissue. Stem Cell Research & Therapy. 2023, 14(1): 291. (CC BY 4.0)

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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