Overview of Clostridiaceae

Ruminococcaceae are obligate anaerobes known for their diverse morphological forms, ranging from rod-shaped to cocci. Within this family, Faecalibacterium prausnitzii is particularly notable for its abundant presence as a commensal bacterium in the human gut microbiota, alongside several Ruminococcus strains, which also inhabit the human gastrointestinal tract. Ruminococcaceae play a significant role as a part of the Firmicutes phylum, which dominates the human gut microbiota, comprising 50-80% of the taxonomic entities and over 84% of the active constituent fraction. Among the Firmicutes, Ruminococcaceae is one of the most prolific families in gut ecosystems, constituting around 30% of the phylotypic varieties. Their prevalence and functional diversity make Ruminococcaceae crucial components of human gut microbiota, influencing various metabolic and physiological processes.

Unlocking the Microbial Keys to Obesity Management

Considering the substantial presence and potential health implications of Ruminococcaceae, Protheragen provides colonization studies to elucidate the role of these bacteria in obesity. Through an exploration of the colonization patterns and metabolic interactions within obese individuals, we seek to unravel their contributions to metabolic regulation and to identify novel therapeutic strategies for addressing obesity and its related complications. By exploring the colonization patterns and metabolic interactions within obese individuals, we seek to unravel their contributions to metabolic regulation and identify novel therapeutic strategies for addressing obesity and its related complications.

Our Ruminococcaceae colonization studies for investigating obesity follow a systematic and comprehensive process:

Animal Preparation

We maintain germ-free male mice in gnotobiotic isolators, feeding them an autoclaved low-fat, polysaccharide-rich chow diet. Ruminococcaceae are introduced into the germ-free mice via gavage to establish gut microbiota. After weaning, littermates are housed individually and fed various diets, including a chow diet, a high-fat/high-sugar Western diet, a fat-restricted diet, or a carbohydrate-restricted diet, to study the effects of diet on microbiota composition and obesity.

Microbiota Analysis

Perform sequencing of the 16S rRNA gene or metagenomic analysis to identify and quantify the presence of Ruminococcaceae family members in the fecal samples.

Fat Content Measurement

The recipient mice are anesthetized at specific time points post-colonization for measurement of total body fat content using dual-energy X-ray absorptiometry (DEXA).

Metabolic Assessments

Conduct metabolic tests such as glucose tolerance tests, insulin tolerance tests, and measurement of serum lipid levels to evaluate the impact of Ruminococcaceae colonization on glucose and lipid metabolism.

Pathological Analysis

After the study duration, sacrifice the mice and perform histological analysis of fat tissue to assess changes in adipocyte size, inflammation, and other pathological features related to obesity.

Workflow

Applications

• Our services can be used to research the composition and function of Ruminococcaceae within the gut microbiome to understand their role in human and animal health.
• Our services can be applied to developing strategies to modulate Ruminococcaceae populations to promote weight loss and manage obesity-related conditions.
• Our services are useful for studying the role of Ruminococcaceae in chronic inflammatory conditions like inflammatory bowel disease (IBD) and its impact on systemic inflammation.

Advantages

• By maintaining germ-free mice in gnotobiotic isolators and feeding them autoclaved diets, we ensure a highly controlled and sterile environment, allowing for a precise study of the effects of Ruminococcaceae colonization on obesity and metabolic health.
• Our studies involve not only microbiota analysis but also comprehensive metabolic assessments, fat content measurements using DEXA, and pathological analysis of fat tissue, offering a holistic view of the impact of Ruminococcaceae on obesity.
• By feeding mice various diets, including chow, high-fat/high-sugar Western diets, and restricted diets, we explore how different nutritional environments influence Ruminococcaceae colonization and its metabolic outcomes.

Publication Data

Frequently Asked Questions

1. What dietary components can Protheragen analyze in the gut microbiota?
   • Effect analysis of high-fat diet on the gut microbiota
   • Effect analysis of a high-carbohydrate diet on the gut microbiota
   • Effect analysis of high-protein diet on the gut microbiota
   • Effect analysis of high-fiber diet on the gut microbiota
   • Effect analysis of calorie-restricted diet on the gut microbiota
   • Effect analysis of the ketogenic diet on the gut microbiota
   • Effect analysis of Mediterranean diet on the gut microbiota
2. What analyses can we perform on adipocyte tissue in obese mice?
   • Adipocyte Size and Count Analysis Service
   • Adipose Tissue Expansion Analysis Service
   • Adipose Tissue Inflammation Analysis Service
   • Adipose Tissue Fibrosis Analysis Service

Protheragen systematically investigates the role of diverse Obesity Microbiota in obesity by establishing these bacteria in germ-free mice, subjecting them to various diets, and analyzing changes in gut microbiota composition, fat content, metabolic parameters, and adipose tissue pathology. If you are interested in the comprehensive approach that aims to elucidate the Contributions of Obesity Microbiota to metabolic regulation and identify potential therapeutic strategies for obesity and related complications, contact us!

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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