Lactobacillus Colonization Studies
InquiryOverview of Lactobacillus Colonization
Gut microorganisms play a crucial role in maintaining the health of the body and are closely related to the body's immune regulation, nutritional digestion, and biological antagonism. The intestinal flora always maintains a dynamic balance between beneficial and harmful microorganisms, which makes the intestinal microecology maintain a favorable environment for a long time. Lactobacillus grows mainly in carbohydrate-rich environments, including milk, meat, cereals, plants, and mucosal surfaces of animals and humans (oral cavity, gastrointestinal tract, and reproductive tract). Through our specialized Obesity Microbiota Research Service, Protheragen aims to delve deeper into the complex relationship between microbial communities and obesity. We help clients gain a comprehensive understanding of Obesity Microbiota Colonization and reveal the mechanisms of its impact. Meanwhile, our Lactobacillus colonization research focuses on optimizing the intestinal microecological balance and evaluating the potential of Lactobacillus in ameliorating obesity and related metabolic problems.
An In-depth Exploration of the Relationship Between Lactobacillus and Obesity
Screening of Lactobacillus
Our company provides professional Lactobacillus screening services, including isolation and purification of the strain, catalase test, Gram stain microscopy, and 16S rRNA gene sequencing. In addition, we evaluate the probiotic properties and safety of Lactobacillus.
- Acid and bile salt tolerance
- Bacteriostatic capacity of fermentation products
- Safety determination
- Gastrointestinal fluid resistance
- Lactobacillus bile salt hydrolase production assay
- Strain adhesion test
Animal Modeling and Metabolic Profiling
Our company uses high-quality mice to construct diet-induced obesity models. Our researchers analyze the intestinal flora and hepatic metabolism of mice on high-fat diets by administering Lactobacillus orally to analyze the mechanisms of the effects on gut flora and hepatic metabolism in mice on high-fat diets.
- Detection of biochemical indicators
We provide serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) measurement services in mice. Moreover, we provide serum insulin measurement services. - Histomorphometric analysis
We use hematoxylin-eosin staining (H&E) to morphologically analyze epididymal adipose and liver tissues in mice. - Basic indicator testing
Our company provides services to analyze the effect of Lactobacillus on the body weight of the mouse, the effect of Lactobacillus on food intake and food utilization, the effect of Lactobacillus on organ mass and organ index, and the effect of Lactobacillus on fat mass and fat coefficient of the mouse. - Changes in gut flora and metabolomic analysis
Our researchers analyze the changes in the intestinal flora of mice after Lactobacillus intervention by 16S rRNA sequencing of the intestinal flora. Our researchers explore the effect of Lactobacillus on mouse liver metabolism and the expression of its metabolites by performing metabolomic analysis of mouse livers.
Workflow
Applications
- Lactobacillus colonization studies' effect on the gut flora and consequent metabolic disturbances are important for exploring mechanisms of intervention in obesity.
- Lactobacillus colonization studies can be used to screen for Lactobacillus species with specific functions.
- Lactobacillus colonization studies lay the foundation for obesity mechanism studies and pathway studies.
- Lactobacillus colonization studies can be used to develop lipid-lowering drugs as well as better prevention and treatment of fatty liver disease.
Advantages of Us
- Our company has abundant animal resources and a team of well-trained personnel to meet the multifaceted research needs of our clients.
- Our company has an efficient data processing team to help clients quickly analyze and organize the results of experiments.
- We provide not only animal models but also cell models such as 3T3-L1 cells and HepG2 cells. Our researchers comprehensively evaluated the effects of Lactobacillus on the expression of genes related to cellular lipid metabolism.
Your First Choice in Anti-Obesity Research
Our company offers an extremely comprehensive and in-depth anti-obesity research service that encompasses the exploration and development of anti-obesity targets, in-depth studies of anti-obesity therapeutics, as well as a rigorous preclinical research phase.
Publication Data
Technology: Enzyme-linked immunosorbent assay (ELISA), rRNA quantitative PCR analysis, Bacterial community analysis
Journal: Microbiology Spectrum
Published: 2021
IF: 3.7
Results: This article focuses on the association between oxidative stress induced by a high-fat diet (HFD) and increased colonization by Lactobacillus sakei. It was found that a high-fat diet induced oxidative stress in experimental animals or people, as evidenced by elevated levels of oxidative stress markers in vivo. L. sakei colonization was significantly increased in high-fat diet-induced obese people. There was a positive correlation between the level of oxidative stress and the number of Lactococcus lactis colonization, suggesting that high-fat diet-induced oxidative stress promotes the colonization of L. lactis in the intestine.
Fig.1 Association between L. sakei colonization and high fat intake with increased gut oxidative stress. (Lee, et al., 2021)
Frequently Asked Questions
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What function does Lactobacillus play in the fight against obesity?
Lactobacillus, especially Lactobacillus griseus and Lactobacillus amyloliquefaciens, are beneficial for weight loss in overweight individuals. They regulate gut flora, reduce body weight and fat mass, and also reduce food intake by decreasing intestinal permeability, reducing inflammation, increasing hypothalamic insulin sensitivity, and increasing satiety. Studies have also shown that L. griseus reduces body mass index, waist circumference, and visceral fat, helping to prevent insulin resistance, metabolic risk, and diabetes.
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What are the research methods for Lactobacillus colonization?
- Plate counting method
- Fluorescent labeling
- Polymerase chain reaction
Protheragen is committed to solving all problems encountered by our clients in gut flora and obesity research. We are always passionate about serving you. Please feel free to contact us.
Reference
- Lee, J.Y.; et al. High-fat-diet-induced oxidative stress linked to the increased colonization of Lactobacillus sakei in an obese population. Microbiology Spectrum. 2021, 9(1): 10-1128. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.