Enterobacteriaceae Colonization Studies
InquiryOverview of Enterobacteriaceae
Intestinal flora is involved in the metabolism of many nutrients in the body, such as sugars and lipids. When the intestinal flora is disturbed, it causes insulin resistance, accumulation of adipose tissue, and metabolic abnormalities, which lead to the development of metabolic diseases such as obesity. Enterobacteriaceae belong to the next classification of Gram-negative bacteria. It consists of more than 30 genera and over 100 species. The Enterobacteriaceae include many harmless commensal bacteria, as well as many pathogens such as Escherichia coli, Salmonella, Shigella, and Klebsiella. The relationship between Enterobacteriaceae and obesity is complex and involves multiple mechanisms and factors. Therefore, Protheragen provides further studies to determine the role of specific Enterobacteriaceae bacteria in the development of obesity and to explore possible therapeutic targets.
In-depth Analysis of the Relationship between Enterobacteriaceae Colonization and Obesity
Animals and Experimental Design
Our researchers provide sterile mouse feeding and Enterobacteriaceae transplantation services. After the transplantation period, we provide a quantitative analysis of Enterobacteriaceae and an analysis of changes in relative abundance. The analytical methods that we commonly use include flow cytometry, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), 16S rRNA sequencing, and other analytical methods.
Basic Testing
Our researchers have accumulated years of experience in colony transplantation and subsequent testing. We offer testing for several indicators, including but not limited to body weight, fat weight, tissue fat content, body fat distribution, subcutaneous fat content, and a range of blood biochemical indicators.
Intestinal Histological and Immunohistochemistry Examination
We offer professional histopathological change analysis services by staining tissue sections. Five fields of view per section are selected for counting to ensure accuracy.
Workflow
Applications
- Enterobacteriaceae colonization studies can be used for a comprehensive assessment to study the role of obesity in bacterial infections.
- Enterobacteriaceae colonization studies can be used to elucidate the role of specific Enterobacteriaceae families in animal models.
- Enterobacteriaceae colonization studies can be used for the analysis of interactions between the intestinal immune system and pathogen defenses.
Advantages of Us
- Our company customizes research protocols according to the client's specific needs, including experimental design, sample collection, data analysis, and interpretation of results to ensure that research objectives are effectively achieved.
- Our company has a team of experts in the fields of microbiology, immunology, bioinformatics, nutrition, and metabolism who have an in-depth understanding and experience in research related to gut flora and obesity.
- Our company provides translational research from basic science to preclinical studies, including the study of gut flora-obesity interactions in animal models, and provides the basis for clinical studies.
Fighting Obesity: From Molecular Mechanisms to Preclinical Research
In the fight against obesity, a global health challenge, we are committed to providing a one-stop solution from the in-depth exploration of its complex molecular mechanisms to the rigorous preclinical research phase. This solution covers not only the precise development and optimization of molecular targets to target key biomolecular pathways affecting weight regulation, energy metabolism, and fat accumulation but also the development of cutting-edge molecular therapeutics aimed at regulating specific signaling pathways or enhancing the body's metabolic capacity to achieve safe and effective weight management.
To validate the efficacy and safety of these targets and therapies, it is crucial to construct animal models that accurately mimic the pathophysiological characteristics of human obesity. These models not only help us to deeply understand the multifactorial interactions in the development of obesity but also lay a solid foundation for the subsequent pharmacological evaluation. We systematically evaluate the efficacy, pharmacokinetic properties, safety, and potential side effects of candidate drugs or therapies, ensuring that the highest standards of rigor are followed at every step.
Publication Data
Technology: Flow cytometry, Microscope, Staining, Immunohistochemistry
Journal: Frontiers in Veterinary Science
Published: 2022
IF: 2.6
Results: This article focuses on the differences in intestinal immune responses between obese and non-obese mice after a challenge with E. coli. There were no significant differences in intestinal T cell subsets in obese mice compared to normal mice when they were not infected with E. coli. After the E. coli challenge, intestinal T-cell subsets in obese mice exhibited a more active immune response, particularly the ratio of CD4+ T cells to CD8+ T cells. The study suggests that obese mice have more active intestinal mucosal immune responses after E. coli infection, which may be related to the altered number and function of immune cells in obese mice.
Fig.1 Comparison between control and obese groups after E. coli infection. (Cai, et al., 2022)
Frequently Asked Questions
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What are the commonly used DNA extraction methods?
Our common DNA extraction methods include the boiling method, lye heating and cracking method, Chelex-100 extraction, and kit DNA extraction.
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What are the common methods of fecal microbiota transplantation?
Three methods are often used:
- Introduction of the bacterial solution into the end of the small intestine through enteroscopy
- Delivery of bacterial solution into the jejunum through a jejunal nutritional tube or a nasojejunal nutritional tube
- Enema
With our deep scientific expertise, advanced tools, and interdisciplinary team of professionals, Protheragen is your indispensable partner in the field of anti-obesity research. Please feel free to contact us to customize your research route.
Reference
- Cai, D.; et al. More active intestinal immunity developed by obese mice than non-obese mice after being challenged by Escherichia coli. Frontiers in Veterinary Science. 2022, 9: 851226. (CC BY 4.0)
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.