Overview of Glucose Metabolism

The process of glucose metabolism plays a crucial role in maintaining the body's normal physiological function. When glucose metabolism is disturbed, it leads to a series of health problems. Protheragen provides analysis of glucose metabolism-related molecular mechanisms and potential gene targets to help clients understand the complex relationship between obesity and Sugar-related Molecular Metabolism.

Screening and Validation of Glucose Metabolism Factors in Obesity

Glucose catabolism is a complex network involving multiple metabolites, and each metabolite is involved in various metabolic pathways. We have a well-trained biological team to provide fast and accurate glucose metabolism analysis services to our clients.

Stable Isotope-Resolved Metabolomics (SIRM)

• Our company deduces glucose metabolic pathways and fluxes by analyzing the law of change from stable isotope tracing of precursors to products. Combined with nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis, we help clients discover the metabolic pathways of metabolites and reveal the changing patterns of metabolic fluxes.
• Our researchers provide ¹³C labeling of precursors of glucose catabolism and quantitative analysis of the isotopic distribution of their downstream metabolites. Moreover, our service process includes the selection of stable isotope tracers, the introduction of stable isotope tracers, the collection and processing of samples, the analysis of data, and the analysis of glucose metabolic pathways.

Bioluminescent Imaging (BLI)

Based on the bioorthogonal click reaction between activatable caged fluorescein triaryl phosphate and azide-modified glucose molecules, our researchers provide quantitative detection of bioluminescent signals. The higher the light intensity, the higher the level of glucose metabolism.

Bioinformatics Approach

Our company provides genetic analysis of glucose metabolism-related genes using a bioinformatics approach. Moreover, we validate using in vitro cellular experiments to provide new ideas for obesity therapies based on a glucose metabolism perspective.

Metabolite Analysis

For glucose metabolites from different sample sources, we provide professional extraction, separation, and characterization services. Our researchers use organic reagents to extract the samples, which are freeze-dried and then subjected to a two-step derivatization for analysis by gas chromatography coupled to triple quadrupole mass spectrometry (GC-MS/MS).

• For metabolites with low content, our researchers optimize them to obtain the ion pair with the best abundance as the quantitative ion pair to improve the sensitivity.
• For high-content metabolites, our researchers optimize the ion pair with low abundance as the quantitative ion pair to broaden its linear range.

Workflow

Applications

• Glucose metabolism analysis provides a new theoretical basis and intervention targets for early prevention and treatment of glucose metabolism-related diseases.
• Studying the metabolic pattern of glucose catabolism in vivo provides ideas and methods for the pathogenesis of diseases related to glucose catabolism disorders and the development of drugs.
• Glucose metabolism analysis can be used to study the pathogenesis of obesity and screen potential therapeutic targets.

Advantages of Us

• Our metabolite analysis service analyzes both high and low-abundance metabolites with simple pre-treatment, high sensitivity, good reproducibility, and a wide linear range.
• Our SIRM service not only tracks the metabolic pattern of labeled compounds in vivo through NMR and mass spectrometry but also determines the specific metabolic pathways by using the distribution of isotopic peaks of intermediate metabolites.
• Our BLI service has the advantages of high specificity, no autofluorescence, high sensitivity, accurate quantification, etc. It has irreplaceable advantages in real-time observation of disease development and response to drug therapy.

Join Us for an In-Depth Discussion on Obesity

Our company has a group of scientists who are passionate about obesity research. Whether you want to gain a deeper understanding of the root causes of obesity or are looking for potential anti-obesity targets, we support you. We offer services covering every aspect of obesity research, from target screening and therapy development to preclinical studies. We provide you with customized solutions.

Publication Data

Frequently Asked Questions

1. What diseases are closely associated with disorders of glucose metabolism?

   Disorders of glucose catabolism affect the normal functioning of important physiological systems such as the cardiovascular, digestive, and immune systems, leading to obesity, diabetes, osteoarthritis, glycogen storage disease, and neurodegenerative diseases. Importantly, the pathways involved in glucose catabolism can be used as therapeutic targets for diseases. For example, glycolysis is an important target for antitumor drugs, and inhibition of the pentose phosphate pathway has been shown to have a positive effect in the treatment of type 2 diabetes. If you are interested in potential metabolic targets for anti-obesity therapies, our professional researchers provide target discovery and identification services.

2. In which glucose metabolism pathways does SIRM play a role?

   SIRM can be used in the glycolysis pathway, tricarboxylic acid cycle pathway, pentose phosphate pathway, and gluconeogenesis pathway.

Protheragen provides high-quality glucose metabolism analysis services to clients. We look forward to working with you to explore the mysteries of the pathobiology of obesity and to provide you with the best possible service. Please feel free to contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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