Obesity Protein Array Service
InquiryObesity is a multifaceted metabolic disorder characterized by the dysregulation of numerous secreted factors, including adipokines, cytokines, and growth factors. These molecules act as critical mediators of inter-organ communication, influencing insulin sensitivity, inflammation, and energy homeostasis.
Obesity Protein Array Service for Preclinical Research
Protheragen offers a high-throughput, multiplexed obesity protein array service specifically designed to profile these markers in preclinical models. By simultaneously detecting dozens of obesity-related targets in a single sample, our service provides a holistic view of the proteomic landscape, enabling researchers to identify biomarkers and elucidate mechanisms of action for novel drug candidates.
Core Technologies
Our platform leverages advanced chemiluminescent antibody array technology and reverse-phase protein array (RPPA) methodologies.
- Sandwich-Based Membrane Arrays
Utilizing high-affinity capture antibodies spotted onto nitrocellulose membranes, we achieve sensitive detection of soluble mediators (e.g., adiponectin, leptin, IL-6) through biotin-streptavidin-HRP signal amplification.
- High-Density Microarrays
For larger-scale screening, our glass-slide-based arrays allow for the parallel quantification of up to 60+ targets with minimal sample volume, ensuring robust data even with limited preclinical specimens like mouse serum or tissue lysates.
- Validation Protocols
Every array is supported by rigorous cross-reactivity testing and internal controls to ensure that the signals detected are specific and reproducible across different experimental batches.
Service Scope
Our service is optimized for a wide range of preclinical biological samples:
- Species
Human, mouse, and rat-specific arrays.
- Sample Types
Serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates (e.g., white/brown adipose tissue, liver, muscle).
- Target Diversity
Includes adipokines (leptin, adiponectin, resistin), inflammatory cytokines (TNF-α, IL-1β), metabolic regulators (Insulin, glucagon, FGF-21), and angiogenesis factors (VEGF).
Discuss Your Preclinical Workflow with Our Specialists.
Workflow
Protheragen provides a streamlined, end-to-end service to ensure your preclinical data is delivered with precision and speed.
Fields of Application
Our obesity protein array service serves as a versatile analytical engine, bridging the gap between high-volume screening and deep mechanistic insight across diverse areas of metabolic investigation.
- Drug Discovery: Screening lead compounds for their ability to modulate adipokine secretion and reduce systemic inflammation.
- Biomarker Identification: Discovering novel circulating protein signatures that correlate with weight loss or improved metabolic health in Animal Models.
- Mechanism of Action (MoA) Studies: Understanding how a specific genetic knockout or pharmacological agent reshapes the adipose tissue secretome.
- Nutraceutical Testing: Evaluating the efficacy of functional foods and supplements in managing diet-induced obesity (DIO).
Advantages
Protheragen stands at the forefront of metabolic proteomics by offering:
High Sensitivity & Dynamic Range
Our signal amplification techniques enable the detection of low-abundance cytokines and signaling proteins that typically fall below the noise floor of standard mass spectrometry. By expanding the dynamic range to over 5–6 logs, we ensure that both high-titer metabolic markers and trace-level regulatory proteins are captured in a single assay, providing a truly holistic view of the proteome.
Minimal Sample Requirement
We recognize that sample volume is often the primary bottleneck in translational research. Our optimized micro-scale workflows require as little as 50 µL of serum or 200 µg of total protein lysate. This efficiency is critical for longitudinal studies, allowing for repeated sampling from the same small animal models (e.g., mice) without compromising their physiological stability or the study’s statistical power.
Rapid Turnaround
In the fast-paced world of drug development, timing is everything. Protheragen’s streamlined laboratory pipeline reduces the gap between hypothesis and verification. From sample receipt to the delivery of a detailed report, our standard processing time is typically 2–3 weeks, allowing your team to maintain momentum in iterative experimental cycles.
Expert Data Interpretation
We provide more than just a spreadsheet of protein concentrations. Every project is overseen by PhD-level scientists who perform advanced bioinformatic analysis, including pathway enrichment and network mapping. We transform raw data into actionable insights, helping you visualize exactly how your therapeutic intervention modulates specific metabolic and inflammatory pathways.
Proven Reliability
Accuracy is our baseline. Our methodologies are backed by rigorous internal validation and a track record of success in high-impact research. Our results consistently demonstrate a high correlation (R2 > 0.90) with gold-standard ELISA validation, ensuring that the data you receive from our arrays is both robust and highly reproducible.
Inquire Now to Receive a Custom Quote and Project Feasibility Assessment.
Customer Review
Driving Efficiency in Lead Optimization
"Working with Protheragen significantly accelerated our lead optimization phase. Their obesity protein array allowed us to see that our compound wasn't just reducing fat mass, but was actively shifting the adipokine profile from pro-inflammatory to insulin-sensitizing. The data was clean, and the report was ready for our internal board review within two weeks."
Dr. D. G., Cardiovascular and Metabolic Research
Exceptional Support for Complex Tissue Analysis
"The technical support at Protheragen is exceptional. They helped us troubleshoot our tissue homogenization process, which was tricky with fibrotic adipose samples. Their multiplex approach saved us months of work compared to running individual ELISAs. We look forward to our next collaboration on our NASH project."
Mr. P. R., Academic Research Institute
Frequently Asked Questions
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What is the minimum volume of mouse serum required?
We typically require 50–100 µL of serum for a full obesity array, though we can often optimize for smaller volumes.
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Can you detect phosphorylated proteins involved in obesity signaling?
Yes, our RPPA-based options include targets like p-AKT, p-ERK, and p-AMPK.
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How do arrays compare to individual ELISAs?
Arrays are much more cost-effective and sample-efficient when you need to look at more than 3–5 targets simultaneously.
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Are your services suitable for human clinical trials?
No, our services are strictly for preclinical research and are not for diagnostic use.
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Do you provide help with sample extraction?
Absolutely. We provide validated lysis buffer recipes and step-by-step instructions.
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Can I customize the list of proteins on the array?
We offer several pre-validated panels, but we can also discuss custom printing for high-volume projects.
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Is the data quantitative or semi-quantitative?
Most of our membrane arrays provide relative quantification (fold-change), while our glass-slide arrays can provide absolute concentrations using a standard curve.
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What species do you support?
We have dedicated panels for human, mouse, and rat. Cross-reactivity with other species can be tested upon request.
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How do I interpret the heatmap in my report?
Our reports include a guide on how to read the statistical significance and clustering of your samples.
Contact Us
Protheragen provides a robust, high-throughput solution for profiling the complex protein networks associated with obesity and metabolic dysfunction. Our expertise in preclinical proteomics ensures that your drug discovery and basic research projects are supported by high-quality, actionable data.
Contact Protheragen for More Information and to Discuss Your Project
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.