AdipoUpX™ Human KSR2 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human KSR2 MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human KSR2 gene. KSR2 is a molecular scaffold that regulates AMP-activated protein kinase (AMPK) and Raf/MEK/ERK signaling, making it a major driver of basal metabolic rate. By utilizing a vector optimized for stem cells and other mammalian cell lines, this product ensures robust, long-term expression for metabolic signaling research. Product quality is ensured by rigorous purification and comprehensive QC screening against mycoplasma and contaminants.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: KSR2

Full Name: Kinase suppressor of ras 2

Gene ID: 283455

RefSeq ID-1: NP_775869.3

RefSeq ID-2: NM_173598.4

Summary: KSR2 encodes a serine/threonine kinase scaffold that participates in Ras signaling, calcium-mediated pathways, and the positive regulation of cold-induced thermogenesis. It functions upstream in the MAPK signaling cascade and is active in both the cytosol and plasma membrane, making it a key regulator of energy homeostasis and thermogenic processes.

AdipoUpX™ Human KSR2 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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