Product Overview
Description:
AdipoUpX™ Human INPP5E MSCV Particle (Overexpression) is a high-efficiency retroviral tool engineered for stable overexpression of the human INPP5E gene. INPP5E regulates phosphoinositide signaling, impacting ciliary function and metabolic signaling pathways. The MSCV backbone enables long-term expression in hematopoietic stem cells, stem cells, and mammalian models. Each batch undergoes sterility, titer, and mycoplasma testing.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
INPP5E
Full Name:
Inositol polyphosphate-5-phosphatase E
Gene Symbol:
CPD4; CORS1; JBTS1; MORMS; PPI5PIV; pharbin
Summary:
INPP5E encodes an inositol polyphosphate 5-phosphatase that regulates phosphoinositide signaling by hydrolyzing key second messengers such as Ins(1,4,5)P3. It is also involved in regulating Golgi-associated membrane trafficking and cellular signaling pathways. Mutations in this gene are linked to Joubert syndrome and related ciliopathies affecting multiple organ systems.
