Product Overview
Description:
AdipoUpX™ Human TUB MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle designed for the stable overexpression of the human TUB gene. The Tubby protein is a key transcription factor associated with late-onset obesity and neuronal maintenance. Its structure is optimized for stem cells, hematopoietic stem cells, etc., providing robust gene integration for longitudinal metabolic research. This system enables rapid validation of TUB's effects on energy balance in cellular models. Product quality is ensured by comprehensive quality control screening against contaminants.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
TUB
Full Name:
TUB bipartite transcription factor
Gene Symbol:
rd5; RDOB
Summary:
TUB is a member of the Tubby family of bipartite transcription factors. This protein serves as a unique membrane-bound transcription regulator that remains anchored to the plasma membrane until specific signaling events—namely phosphoinositide hydrolysis—trigger its release. Once released, it translocates to the nucleus to regulate gene expression. Defects in this translocation process or the protein itself are implicated in obesity and progressive sensorineural loss (such as retinal and hearing degradation), marking it as a key link between cellular signaling and metabolic homeostasis.
