Product Overview
Description:
AdipoUpX™ Human BBIP1 MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human BBIP1 (BBS18) gene. It plays a structural role in the assembly of the BBSome, making it essential for studying the molecular basis of Bardet-Biedl syndrome. The vector is optimized for stem cells, hematopoietic stem cells, and other lineages, facilitating stable, high-level expression. Product quality is ensured by rigorous purification, titer validation, and comprehensive QC screening to ensure experimental results are safe and accurate.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
BBIP1
Full Name:
BBSome interacting protein 1
Gene Symbol:
BBS18; BBIP10; bA348N5.3; NCRNA00081
Summary:
BBIP1 (also known as BBS18) provides a critical structural link required for the assembly of the BBSome complex. Without this protein, the BBSome cannot form, leading to a total failure in the trafficking of G-protein-coupled receptors to and from the primary cilium. This molecular failure results in Bardet-Biedl Syndrome 18. Like other forms of BBS, this condition is characterized by a high risk of severe obesity, as well as vision loss and kidney anomalies, underscoring the absolute necessity of BBIP1 for ciliary-mediated metabolic control.
