AdipoUpX™ Human PPARGC1A MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PPARGC1A MSCV Particle (Overexpression) is a powerful gene delivery tool designed for the long-term stable overexpression of the human PPARGC1A (PGC-1α) gene. As a master regulator of mitochondrial biogenesis and thermogenesis, its stable expression is essential for studying metabolic health. The MSCV backbone is optimized for stem cells, hematopoietic stem cells, etc., ensuring high-level gene integration across diverse lineages. This product undergoes rigorous testing for titer, sterility, and mycoplasma to guarantee experimental reliability.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PPARGC1A

Full Name: PPARG coactivator 1 alpha

Gene Symbol: LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)

Gene ID: 10891

RefSeq ID-1: NP_001317680.1

RefSeq ID-2: NM_001330751.2

Summary: PPARGC1A encodes a transcriptional coactivator that regulates genes involved in energy metabolism and mitochondrial biogenesis. It interacts with PPARγ as well as other transcription factors, including CREB and nuclear respiratory factors (NRFs), linking external stimuli to metabolic regulation. PPARGC1A also influences muscle fiber type determination, cholesterol homeostasis, and may contribute to obesity development.

AdipoUpX™ Human PPARGC1A MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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