Product Overview
Description:
AdipoUpX™ Human KCNJ11 MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle professionally engineered for the stable expression of the human KCNJ11 gene. It encodes the Kir6.2 subunit of the ATP-sensitive potassium channel, a key target in insulin secretion research. The MSCV structure is optimized for stem cells, hematopoietic stem cells, etc., ensuring stable, high-level gene integration and expression. Every batch passes through exhaustive quality control—including titer and mycoplasma detection—to ensure research reliability and safety.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
KCNJ11
Full Name:
Potassium inwardly rectifying channel subfamily J member 11
Gene Symbol:
BIR; HHF2; PHHI; IKATP; PNDM2; TNDM3; KIR6.2; MODY13
Summary:
KCNJ11 encodes an inward-rectifier potassium channel that forms part of the ATP-sensitive K⁺ channel complex in pancreatic β-cells. It regulates insulin secretion in response to metabolic signals and associates with the sulfonylurea receptor SUR. Mutations can cause hyperinsulinemic hypoglycemia of infancy, neonatal diabetes, and contribute to type 2 diabetes, indirectly affecting body weight regulation. Multiple alternatively spliced isoforms exist.
