Product Overview
Description:
AdipoUpX™ Human ICAM1 MSCV Particle (Overexpression) is an optimized MSCV particle for the stable and efficient expression of intercellular adhesion molecule 1. This tool is ideal for establishing cellular models to study the recruitment of inflammatory cells into adipose tissue during the development of obesity. The vector is compatible with hematopoietic stem cells, stem cells, and various mammalian cell lines, ensuring robust gene integration. Comprehensive quality control, including sterility and titer verification, ensures that this product meets the highest standards for experimental consistency and safety.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
ICAM1
Full Name:
Intercellular adhesion molecule 1
Gene Symbol:
BB2; CD54; P3.58
Summary:
ICAM1 is a cell surface glycoprotein typically expressed on endothelial and immune system cells. It facilitates leukocyte adhesion by binding to specific integrins and is also utilized as an entry point for certain viruses. In obesity, its elevated expression is often a biomarker for the chronic vascular inflammation associated with metabolic syndrome.
