Product Overview
Description:
AdipoUpX™ Human IL1B MSCV Particle (Overexpression) is a high-titer retroviral tool designed for the stable expression of interleukin-1 beta, a master regulator of the inflammatory response. This product is essential for researchers investigating the link between chronic low-grade inflammation in adipose tissue and the development of insulin resistance. Optimized for hematopoietic stem cells, stem cells, and diverse mammalian cell lines, this MSCV-based system ensures high-level integration and long-term expression. Every batch undergoes comprehensive quality control, including titer, sterility, and mycoplasma testing, to guarantee experimental safety and reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
IL1B
Full Name:
Interleukin 1 beta
Gene Symbol:
IL-1; IL1F2; IL1beta; IL1-BETA
Summary:
IL1B encodes a cytokine belonging to the interleukin-1 family, produced as an inactive proprotein by activated macrophages and activated by caspase-1. This cytokine plays a central role in inflammation and regulates cellular processes such as proliferation, differentiation, and apoptosis. IL1B contributes to inflammatory pain in the CNS via induction of COX-2 and is implicated in osteoarthritis pathogenesis. Elevated IL1B levels have been observed in severe COVID-19 patients, contributing to lung injury. IL1B is part of a gene cluster with eight other interleukin-1 family members on chromosome 2.
