Product Overview
Description:
AdipoUpX™ Human SREBF1 MSCV Particle (Overexpression) is a specialized retroviral vector designed to drive the stable expression of SREBP-1c, the primary transcriptional activator of de novo lipogenesis. This product is indispensable for investigating fatty acid synthesis and lipid accumulation in various metabolic contexts. Its structure is optimized for high-fidelity integration in stem cells, hematopoietic stem cells, and diverse mammalian cell lines. Each lot is verified through stringent quality control protocols, including sterility and mycoplasma testing, to provide a safe and effective tool for obesity genetics research.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
SREBF1
Full Name:
Sterol regulatory element binding transcription factor 1
Gene Symbol:
HMD; IFAP2; SREBP1; bHLHd1
Summary:
SREBF1 encodes a bHLH-leucine zipper transcription factor that binds sterol regulatory elements to control cholesterol and lipid biosynthesis. The protein is synthesized as a membrane-bound precursor and translocates to the nucleus upon cleavage, which is inhibited by sterols. Alternative promoters and splicing generate multiple transcript variants, including SREBP-1a and SREBP-1c.
