Product Overview
Description:
AdipoUpX™ Human KCTD15 MSCV Particle (Overexpression) is designed for efficient and stable overexpression of the human KCTD15 gene using an optimized MSCV retroviral backbone. KCTD15 has been implicated in appetite regulation and adiposity through its influence on neurodevelopmental and metabolic pathways. This product is suitable for use in hematopoietic stem cells, stem cells, and other relevant mammalian cell models for obesity research. Each batch undergoes rigorous quality control, including viral titer validation, sterility assurance, and mycoplasma testing to ensure reproducibility and biosafety.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
KCTD15
Full Name:
Potassium channel tetramerization domain containing 15
Summary:
The KCTD15 gene produces a protein characterized by its ability to bind to identical units and is localized primarily in the nucleus. It is predicted to function as a negative regulator of DNA-templated transcription, potentially silencing specific genetic programs. Notably, KCTD15 has gained attention in obesity research as several genome-wide association studies have identified it as a significant locus linked to increased Body Mass Index (BMI).
