Product Overview
Description:
AdipoUpX™ Human POMC MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable expression of the human POMC gene. The vector structure has been optimized for hematopoietic and stem cells. POMC generates key peptides like α-MSH, involved in satiety signaling. This stable integration system is an ideal tool for investigating hypothalamic neuropeptide metabolism mechanisms in cellular models. Researchers can study its post-translational modifications and precise role in appetite suppression pathways.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
POMC
Full Name:
Pro-opiomelanocortin
Gene Symbol:
LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH
Summary:
The POMC gene encodes a polypeptide hormone precursor characterized by eight potential cleavage sites. Its biological functions primarily depend on tissue-specific post-translational processing, wherein prohormone convertases recognize and cleave the precursor at specific sites to generate diverse bioactive peptides—up to ten distinct peptides—in different tissues. For instance, the pituitary gland primarily produces ACTH, while the hypothalamus mainly generates appetite-suppressing α-MSH and analgesic endorphins. Molecular defects in POMC lead to severe, early-onset obesity (due to lack of α-MSH signaling), adrenal insufficiency, and distinctive red hair.
