AdipoUpX™ Human PPARG MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PPARG MSCV Particle (Overexpression) is a high-titer, ready-to-use MSCV retroviral particle. It is professionally engineered for the specific and efficient stable overexpression of the human PPARG gene. The vector structure is optimized for hematopoietic and stem cell types, etc. This ensures stable, high-level gene integration and expression within these cell lines, making it an ideal tool for establishing stable cell lines and studying adipocyte differentiation and energy homeostasis. The viral system offers high flexibility for customizing functional components.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PPARG

Full Name: Peroxisome proliferator activated receptor gamma

Gene Symbol: GLM1; CIMT1; FPLD3; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma

Gene ID: 5468

RefSeq ID: NM_005037.5, NP_005028.4

Summary: PPARG codes for the nuclear receptor peroxisome proliferator-activated receptor gamma, which serves as the master transcriptional switch for fatty acid storage and glucose metabolism. The protein must partner with the retinoid X receptor (RXR) to activate target genes crucial for adipocyte differentiation and insulin sensitivity. Due to its central role in regulating lipid and glucose flux, PPARG is a highly validated therapeutic target for type 2 diabetes (through TZD drugs) and is strongly implicated in the molecular pathology of obesity, atherosclerosis, and various cancers. Alternative splicing allows for the production of different protein isoforms.

AdipoUpX™ Human PPARG MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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