Product Overview
Description:
AdipoUpX™ Human BBS5 MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human BBS5 gene. It is involved in the recruitment of the BBSome to the ciliary membrane, a critical step for leptin receptor trafficking. The vector structure is optimized for stem cells and various mammalian cell types, ensuring stable gene integration for functional validation in metabolic disease models. Product quality is ensured by rigorous purification and comprehensive quality control screening against mycoplasma to guarantee experimental integrity.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
BBS5
Full Name:
Bardet-Biedl syndrome 5
Summary:
BBS5 is an integral component of the BBSome complex, specifically required for the complex to bind to certain phospholipids in the ciliary membrane. Its expression is restricted to ciliated cells, where it is mandatory for ciliogenesis (the birth of cilia). Mutations in BBS5 lead to the classic Bardet-Biedl Syndrome phenotype. Because primary cilia are essential for sensing satiety hormones like leptin, the ciliary defects caused by BBS5 mutations lead to profound obesity, alongside retinal dystrophy and polydactyly.
