AdipoUpX™ Human PTEN MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PTEN MSCV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human PTEN gene. PTEN acts as a major negative regulator of the PI3K signaling pathway, influencing lipid metabolism and cellular growth. The vector structure is optimized for stem cells, hematopoietic stem cells, etc., providing a stable platform for in vitro functional validation. Product quality is ensured by rigorous purification and comprehensive quality control, ensuring that your results are repeatable, safe, and accurate.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PTEN

Full Name: Phosphatase and tensin homolog

Gene Symbol: BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; PTENgama

Gene ID: 5728

RefSeq ID-1: NP_000305.3

RefSeq ID-2: NM_000314.6

Summary: PTEN encodes a phosphatase that dephosphorylates phosphoinositides, negatively regulating the AKT signaling pathway and acting as a tumor suppressor. Alternative isoforms, including a longer mitochondrial-associated form, may affect energy metabolism. Dysregulation of PTEN can disrupt insulin signaling and cellular metabolic homeostasis, linking it to obesity susceptibility.

AdipoUpX™ Human PTEN MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products