Product Overview
Description:
AdipoUpX™ Human ALMS1 MSCV Particle (Overexpression) is a high-titer MSCV particle designed for the specific and efficient stable expression of the human ALMS1 gene. Mutations in ALMS1 lead to Alström syndrome, characterized by morbid obesity and insulin resistance. This stable integration system is an ideal tool for investigating ciliary function and metabolic signaling in hematopoietic stem cells, stem cells, etc. To ensure experimental reliability, all viral systems undergo rigorous testing for quality and efficacy across various mammalian cell types.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
ALMS1
Full Name:
ALMS1 centrosome and basal body associated protein
Gene Symbol:
ALSS
Summary:
ALMS1 encodes a protein containing a large tandem-repeat domain and regions of low complexity, crucial for microtubule organization and cilia formation and maintenance. Mutations in ALMS1 cause Alström syndrome, which affects multiple organ systems. The gene has a nearby pseudogene on chromosome 2, and although alternative splice variants exist, the full-length sequences have not yet been fully characterized.
