AdipoUpX™ Human BBS12 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS12 MSCV Particle (Overexpression) is a professionally engineered vector particle providing efficient and stable overexpression of human BBS12. This chaperonin-like protein is crucial for the initial stages of BBSome assembly. The MSCV vector is optimized specifically for stem cells, embryonic cells, and other mammalian cell lines, facilitating the establishment of high-fidelity functional models. All products pass through comprehensive quality control validation to ensure the creation of safe and effective stable cell lines for syndromic obesity research.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: BBS12

Full Name: Bardet-Biedl syndrome 12

Gene Symbol: C4orf24

Gene ID: 166379

RefSeq ID-1: NP_001171478.1

RefSeq ID-2: NM_001178007.2

Summary: BBS12 encodes a chaperonin-like protein that supports protein folding and intracellular membrane trafficking. It is an essential component in the assembly of the BBSome complex and contributes to adipocyte differentiation. Mutations in BBS12 cause Bardet–Biedl syndrome type 12, a ciliopathy associated with obesity, retinal degeneration, and renal abnormalities. Two transcript variants encode the same protein.

AdipoUpX™ Human BBS12 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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