Product Overview
Description:
AdipoUpX™ Human CPT1A MSCV Particle (Overexpression) is a professionally engineered MSCV retroviral particle designed for the stable expression of the carnitine palmitoyltransferase 1A gene. As a key controller of mitochondrial fatty acid beta-oxidation, CPT1A is vital for research focused on energy expenditure and lipid catabolism. The vector is optimized for use in stem cells, hematopoietic stem cells, and varied mammalian cell lines, facilitating the creation of stable metabolic models. Each product includes comprehensive quality control, ensuring high-titer performance and freedom from mycoplasma and microbial contaminants.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
CPT1A
Full Name:
Carnitine palmitoyltransferase 1A
Gene Symbol:
CPT1; CPT I; CPT1-L; CPTI-L; L-CPT1
Summary:
CPT1A encodes carnitine palmitoyltransferase I, a mitochondrial outer membrane enzyme essential for transporting long-chain fatty acids into mitochondria for β-oxidation. Deficiency in CPT1A reduces fatty acid oxidation rates. Alternative splicing produces multiple transcript variants encoding different isoforms.
