Product Overview
Description:
AdipoUpX™ Human EIF4EBP1 MSCV Particle (Overexpression) is a retroviral tool designed for stable overexpression of the human EIF4EBP1 gene. EIF4EBP1 is a key regulator of cap-dependent translation and is closely linked to insulin signaling and metabolic control. The MSCV system enables persistent expression in hematopoietic stem cells, stem cells, and mammalian cell models for translational regulation studies in metabolic contexts. Each batch undergoes rigorous QC, including titer and contamination testing, ensuring experimental reliability.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
EIF4EBP1
Full Name:
Eukaryotic translation initiation factor 4E binding protein 1
Gene Symbol:
BP-1; 4EBP1; 4E-BP1; PHAS-I
Summary:
EIF4EBP1 encodes a translational repressor that binds eIF4E and inhibits assembly of the eIF4F initiation complex. Through this interaction, it suppresses cap-dependent mRNA translation. In response to signaling pathways such as insulin or stress stimuli, phosphorylation of EIF4EBP1 releases eIF4E, allowing translation initiation to proceed. This positions EIF4EBP1 as a key regulator of protein synthesis.
