Product Overview
Description:
AdipoUpX™ Human COL5A2 MSCV Particle (Overexpression) is a retroviral tool designed for stable overexpression of the human COL5A2 gene. COL5A2 is an extracellular matrix component contributing to tissue remodeling and adipose fibrosis in obesity. The MSCV vector supports long-term expression in hematopoietic stem cells, stem cells, and mammalian systems. Each batch is QC-tested for sterility and titer.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.
Sterility Test:
No microbial contamination was detected in any product.
Mycoplasma Test:
No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.
Other QC:
Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.
Storage:
The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.
Stability:
Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.
Shipping Condition:
Our MSCV retroviral products are shipped using dry ice.
Handling Notes:
Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.
Target Profile
Gene Name:
COL5A2
Full Name:
Collagen type V alpha 2 chain
Gene Symbol:
EDSC; EDSCL2
Summary:
COL5A2 encodes the alpha-2 chain of type V collagen, a minor fibrillar collagen that regulates the assembly of type I collagen fibers. It plays an important structural role in connective tissues. Mutations in COL5A2 are associated with connective tissue disorders, including certain forms of Ehlers-Danlos syndrome.
