AdipoUpX™ Human SIM1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SIM1 MSCV Particle (Overexpression) is a stable integration tool designed for the efficient overexpression of the human SIM1 gene. As a transcription factor essential for the development of hypothalamic satiety centers, SIM1 is a primary target in obesity genetics. The vector structure is optimized for high-level expression in hematopoietic stem cells, stem cells, and other mammalian cell lines, facilitating the investigation of neurodevelopmental metabolic pathways. The viral system features a modular design for high customization and is verified through strict quality control, including titer and sterility validation.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: SIM1

Full Name: SIM bHLH transcription factor 1

Gene Symbol: bHLHe14

Gene ID: 6492

RefSeq ID-1: NP_001361698.1

RefSeq ID-2: NM_001374769.1

Summary: SIM1 is one of two human homologs of the Drosophila single-minded gene, which is critically involved in embryonic development. Expression profiling has shown that SIM1 transcripts are primarily detectable in fetal tissues, including the developing kidney. Given the established role of the sim gene during neurogenesis in flies, SIM1 has been proposed to influence human brain development and craniofacial formation. Alterations in SIM1 function have been associated with neurodevelopmental abnormalities and impaired regulation of appetite and body weight, implicating this gene in obesity-related pathways.

AdipoUpX™ Human SIM1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products