AdipoUpX™ Human PDE3B MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PDE3B MSCV Particle (Overexpression) provides stable expression of the human PDE3B gene, a phosphodiesterase regulating cAMP signaling in adipocytes and hepatocytes. PDE3B influences lipolysis, insulin signaling, and energy storage in obesity. The MSCV system enables long-term gene integration in stem cells and other mammalian cell types. Each batch is validated through comprehensive QC assays.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: PDE3B

Full Name: Phosphodiesterase 3B

Gene Symbol: HcGIP1; cGIPDE1

Gene ID: 5140

RefSeq ID-1: NP_000913.2

RefSeq ID-2: NM_000922.3

Summary: PDE3B encodes a cyclic nucleotide phosphodiesterase that hydrolyzes cAMP, thereby modulating intracellular signaling pathways downstream of insulin and catecholamines. PDE3B is highly relevant to adipocytes, where it regulates lipolysis and lipid storage. Dysregulation of PDE3B activity contributes to altered fat metabolism and obesity-associated insulin resistance.

AdipoUpX™ Human PDE3B MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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